IMPACTS OF BISPHENOL-A EXPOSURE DURING LARVAL DEVELOPMENT IN CONTAINER DWELLING MOSQUITOES
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1
Illinois State University, School of Biological Sciences, United States
Endocrine disrupting compounds (EDCs) have been implicated in a variety of biological problems from population decline to various cancers. Bisphenol-A (BPA) is one such endocrine disruptor that is widely used in manufacturing and is nearly ubiquitous in the environment. BPA has been shown to have estrogenic (feminizing) and toxic effects in developing organisms from a wide range of vertebrate taxa; however, little is known about effects of this compound on invertebrates. This study investigates endpoint effects and physiological responses of larval mosquitoes to BPA exposure, and the results will inform our understanding of EDC effects on invertebrates. To study the endpoint effects of BPA, larvae of two species of container-dwelling mosquitoes, Aedes aegypti and Aedes albopictus, were reared individually in one of eight concentrations of BPA (serial dilution; 100ppm-0.01ppb) or in a control lacking BPA. We recorded time to adulthood, survival, mass and sex. We found that BPA did not affect sex ratios, time to adulthood or mass in either species; however there was a significant negative effect of BPA on survival in both species at 100ppm. To assess the larval response to BPA we will test for the presence of BPA metabolites; preliminary trials indicate that a detectable amount of water-soluble metabolites (presumably BPA conjugates) are present in the system 48 hours after exposure. In a larger follow up study, larvae will be initially reared individually in water free of BPA. At each instar, 15 larvae will be exposed to tritiated BPA and after 48 hours we will test for the presence of metabolites using thin-layer chromatography. Understanding how mosquitoes respond to exposure to EDCs may help to inform the conservation or control of other invertebrate species with similar life histories. This could subsequently lead to a better understanding and therefore greater effectiveness of pest control.
Acknowledgements
Supported by EPA STAR fellowship F09G11129 to S.G.C.
This poster was developed under STAR Fellowship Assistance Agreement no. F09G11129 awarded by the U.S. Environmental Protection Agency (EPA). It has not been formally reviewed by EPA. The views expressed in this presentation are solely those of SGC and the EPA does not endorse any products or commercial services mentioned in this poster.
Keywords:
Aedes aegypti,
Aedes albopictus,
bisphenol A,
endocrine disruption,
glucuronyltransferase,
mosquito,
sulfotransferase,
xenobiotic metabolism
Conference:
NASCE 2011: The inaugural meeting of the North American Society for Comparative Endocrinology, Ann Arbor, United States, 13 Jul - 16 Jul, 2011.
Presentation Type:
Poster
Topic:
Endocrine disruption
Citation:
Clairardin
S,
Paitz
R,
Juliano
S and
Bowden
R
(2011). IMPACTS OF BISPHENOL-A EXPOSURE DURING LARVAL DEVELOPMENT IN CONTAINER DWELLING MOSQUITOES.
Front. Endocrinol.
Conference Abstract:
NASCE 2011: The inaugural meeting of the North American Society for Comparative Endocrinology.
doi: 10.3389/conf.fendo.2011.04.00119
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Received:
20 Jul 2011;
Published Online:
09 Aug 2011.
*
Correspondence:
Ms. Sandrine Clairardin, Illinois State University, School of Biological Sciences, Normal, IL, United States, sgclair@ilstu.edu