Multiplex immunoassay for allergens using oligonucleotide tethering
-
1
QUANTISCIENTIFICS LLC, United States
Allergy to cow’s milk affects about 2.5% of all children under age 3. While most infants overcome this allergy, milk intolerance is prevalent in most countries. Milk allergy is often associated with other food allergy, as well as, respiratory allergy.
Microplate-based multiplexed immunoassays are useful for the simultaneous measure of multiple analytes from a single sample in a single well. This allows for the creation of disease-specific panels potentially leading to a more accurate prognosis and/or diagnosis of the disease state. The A-squared (A2) MicroArray System (QuantiScientifics, Irvine, CA, USA) is a microplate-based platform for quantitative multiplexed immunoassays. Arrays of oligonucleotides are covalently attached to the bottom of the plate well. Complementary strands are in turn linked to antibodies or antigens. The prepared oligo-antibody (-antigen) conjugates are pooled and applied to the wells. Through the process of hybridization self-assembly of the hybrids leads to the creation of a diagnostic multiplex panel in each well of the plate. In this study, several milk proteins recognized as allergens were assembled into a multiplex panel using oligonucleotide tethering. Here, purified milk proteins whose epitopes are known to be associated with the allergy, as well as, crude milk allergen extracts are conjugated to oligonucleotides to create the panel. The panel included F2 crude extract, beta-lactoglobulin, alpha-lactalbumin, IgG, bovine serum albumin, lactoferrin, and 3 caseins. 13 patient serums were screened for the presence of specific IgE providing a profile and subsequent identification of principal component allergen.
Keywords:
Oligonucleotides,
arrays,
Multiplex assay,
Allergens,
immunoassays
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Matson
R
(2013). Multiplex immunoassay for allergens using oligonucleotide tethering
.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00052
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
09 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Robert Matson, QUANTISCIENTIFICS LLC, Irvine, California, United States, rsmatson@quantiscientifics.com