The importance of experimental milieu in regulation of macrophage activation
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1
Institute of Biophysics, AS CR v.v.i., Department of Free Radical Pathophysiology, Czechia
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2
University Hospital of Cologne, Germany
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3
Brno University of Technology, Faculty of Electrical Engineering and Communication, Czechia
Background: Macrophages are important source of cytokines and other compounds, which recruit additional cells to sites of infection or tissue injury. Importantly, their functions might be directly controlled by the composition of milieu in their immediate vicinity. Therefore, the analysis of their function under different in vitro as well as “simulated” in vivo condition is of particular interest.
Materials and methods: Mice peritoneal macrophages were stimulated with different activators (e.g. LPS, IFN-γ, TNF-α and interleukins or their combination) under different experimental conditions (in vitro and “simulated” in vivo conditions). Consequently, the physiological functions of macrophages were assessed using different luminometric, spectrophotometric, molecular, and immunohistochemical methods.
Results: Our data demonstrate that there exists crucial difference in activation of macrophages under classical in vitro and “simulated” in vivo conditions. These abnormalities are accompanied by significant changes in inflammatory response of macrophages as well as in activation of intracellular signaling pathways.
Conclusions: The activation of mice peritoneal macrophages is dependent on the composition of milieu in their immediate vicinity and the “classically used” in vitro systems seems to be not suitable for correct analysis of their functions.
Acknowledgements
This work was supported by the Academy of Sciences of the Czech Republic (M200041208, AV0Z50040702), and by The Ministry of Education, Youth and Sports (CZ.1.07/2.3.00/30.0030).
Keywords:
Macrophages,
experimental milieu,
activation,
lipopolysacchride,
Interferon-gamma
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Pekarova
M,
Lojek
A,
Martiskova
H,
Klinke
A,
Rudolph
TK,
Svobodova
B,
Maceckova
M,
Kuchta
R,
Kadlec
J,
Kuchtova
Z and
Kubala
L
(2013). The importance of experimental milieu in regulation of macrophage activation.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00366
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Received:
15 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Michaela Pekarova, Institute of Biophysics, AS CR v.v.i., Department of Free Radical Pathophysiology, Brno, 61265, Czechia, pekarovam@ibp.cz