Immunophenotypes of Burkitt’s lymphoma cell lines alter at different Epstein-Barr Virus (EBV) infection latency types
Irina
Spaka1, 2*,
Artjoms
Spaks3, 4,
Svetlana
Kozireva2,
Jevgenija
Osmjana2,
Madara
Upmane2, 3,
Rita
Birkenfelde2,
Vaira
Kalnina2,
Sandra
Lejniece2, 5, 6 and
Irina
Kholodnyuk2
-
1
Riga Stradiņš University, Latvia
-
2
Riga Stradiņš University, August Kirchenstein Institute of Microbiology and Virology, Latvia
-
3
University of Latvia, Latvia
-
4
Pauls Stradins Clinical University Hospital, Thoracic Surgery Center, Latvia
-
5
Rīga Stradiņš University, Department of Internal diseases, Latvia
-
6
Riga Eastern University Hospital, Chemotherapeutic and Hematological Clinic, Latvia
Epstein-Barr virus (EBV) is involved in the pathogenesis of endemic Burkitt’s lymphoma (BL). EBV-positive BL cell lines initially maintain the original tumor phenotype of EBV infection (latency I, LatI), but most of them drift toward a lymphoblast phenotype of EBV latency III (LatIII) during in vitro culturing.
The aim of this study was to characterize the immunophenotypes of BL cell lines and to verify whether particular cell subsets association with the type of EBV infection.
The phenotype analysis of two EBV-negative and eleven EBV-positive (three of LatI and eight of LatIII) BL cell lines was based on determination of CD19, CD10, CD38, CD27, and CD5 expression pattern by means of multicolor flow cytometry (pFC). The same approach was applied for the characterization of B-cell subpopulations in peripheral blood (PB) of 8 healthy adults. The EBV latency type was defined by RT-PCR using EBV transcript specific primers.
In EBV-negative and LatI BL cell lines all cells displayed the CD19+CD10+CD38+ phenotype. In contrast, in three LatIII cell lines that were established by a single cell cloning, more than 90% of the cells were CD19+CD10−CD38+. Four original BL tumor-derived cell lines of LatIII consisted of two cell subsets, CD19+CD10+ and CD19+CD10−, in proportion of 28%-84% and 17%-72% of the cells, respectively. A fraction of the cells in these four LatIII BL cell lines displays the phenotype of PB circulating mature B cells (CD19+CD10−CD38+ CD27+ CD5−). This observation may suggest that expression of the EBV LatIII genes may promote the differentiation of malignant cells.
Acknowledgements
This work was supported by Swedish Cancer Foundation, Karolinska Institutet (Stockholm, Sweden), Latvian Council of Science, and by European Social Fund grant Nr. 2009/0221/1DP/1.1.1.2.0/09/APIA/VIAA/074 (Latvia).
Keywords:
Epstein-Barr virus (EBV),
Burkitt’s lymphoma (BL),
immunophenotype,
Latency I (Lat I),
Latency III (Lat III)
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune receptors and signaling
Citation:
Spaka
I,
Spaks
A,
Kozireva
S,
Osmjana
J,
Upmane
M,
Birkenfelde
R,
Kalnina
V,
Lejniece
S and
Kholodnyuk
I
(2013). Immunophenotypes of Burkitt’s lymphoma cell lines alter at different Epstein-Barr Virus (EBV) infection latency types.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00399
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Received:
19 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Ms. Irina Spaka, Riga Stradiņš University, Riga, Latvia, irinapichura@yahoo.com