T Cell Dysregulation in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis
Griffith University, School of Medical Sciences, Australia
Griffith University, Griffith Health Institute, Australia
Queensland Health, Australia
Gamma delta (γδ) T lymphocytes comprise about 1–10% of peripheral lymphocytes. Although, functionally distinct from the more diverse αβ T cells, γδ T cells have important roles in pathogenesis and inflammation. γδ T cells secrete cytokines and chemokines essential for regulating adaptive immunity. In diseases such as Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) decreased activity of cytotoxic T cells and elevations in FOXP3 secreting T cells have been reported. Dysregulation in T cell function may be an important component of the CFS/ME immune profile. Hence, the objective of this study was to determine the role of γδ T cells in CFS/ME patients. 20 CFS/ME patients (age=51.8±1.74 years) and 30 non-fatigued controls (age= 53.19±1.35years) were recruited for the study. Inclusion into the CFS/ME group was based on the Centre for Disease Prevention and Control (CDC 1994) criteria definition for CFS/ME. 10mL of whole blood was collected from all participants. The whole blood samples were stained with a cocktail of monoclonal antibodies containing CD4, CD62L, CD27, CD45RA, CD3, CD11a, CD94, V delta-1 and V delta-2. Following which samples were analysed on the flow cytometer. The statistical analysis used to determine differences in the data was ANOVA with significant set at P=0.05. Compared to non-fatigue controls, modifications in cell number were observed in the CFS/ME patients. The results demonstrate that CFS/ME may be characterised by discrepancies in other subsets of T cells.
chronic fatigue syndrome,
T Cell Dysregulation,
γδ T cells,
Cytotoxic T Cells,
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
(2013). T Cell Dysregulation in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis.
15th International Congress of Immunology (ICI).
30 Jun 2013;
22 Aug 2013.
Dr. Ekua W Brenu, Griffith University, School of Medical Sciences, Gold Coast, Queensland, 4222, Australia, email@example.com