Nitro-oleic acid regulates the classical and alternative activation of macrophages
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1
Institute of Biophysics, AS CR, v.v.i., Free Radical Pathophysiology, Czechia
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2
University Hospital of Cologne, Department of Experimental Cardiology, Germany
Nitrated fatty acids (NO2-FAs) are endogenously generated as an adaptive response of organism to oxidative conditions. NO2-FAs were shown to exert significant anti-inflammatory signaling action in immune and vascular cell models; nevertheless, detailed mechanisms of their action in regulation of innate immune responses are not completely understood. Therefore, we investigated the role of nitro-oleic acid (OANO2) in classically and alternatively activated macrophages.
In this study we used the concentration range of 0-2000 nM of OANO2. RAW 264.7 and bone marrow-derived macrophages were treated with OANO2 in the presence of lipopolysaccharide (LPS) and interleukin-4 (IL-4) for different time periods. The following analyses (cytokine array, ELISA, luminol-enhanced chemiluminescence, Griess reaction, Western blot, PCR) were focused on the basic physiological responses of macrophages.
Our results showed that OANO2 significantly regulated the activation of macrophages in a dose-dependent manner. More importantly, changes in the production of pro-and anti-inflammatory mediators were associated with reduced activation of c-Jun N-terminal kinase and expression of nuklear factor-kB and CD36, as well as with increased activation of signal transducer and activator of transcription 6.
In conclusion, NO2-FAs are able to serve as a protective compounds that can effectively down regulate the activation of macrophages. Our results have significant implications since the identification of key regulators of immune functions and information about the mode of their action may provide very important data for future clinical investigations.
Acknowledgements
This work was supported by the Academy of Sciences of the Czech Republic (M200041208, AV0Z50040702).
Keywords:
nitro-oleic acid,
Macrophages,
lipopolysaccharide,
interleukin 4,
Inflammation
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Martiskova
H,
Ambrozova
G,
Kubala
L,
Rudolph
TK,
Klinke
A,
Ravekes
T,
Bino
L,
Navratilova
J,
Kolarova
H,
Gajdova
S and
Pekarova
M
(2013). Nitro-oleic acid regulates the classical and alternative activation of macrophages.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.01155
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Received:
12 Jul 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Michaela Pekarova, Institute of Biophysics, AS CR, v.v.i., Free Radical Pathophysiology, Brno, Czechia, pekarovam@ibp.cz