Population Genetic Structure of red mullet (Mullus barbatus L.) in Turkish Sea Based on Mitochondrial DNA
-
1
Adnan Menderes University, Biology, Türkiye
Aim: Mullus barbatus (red mullet) is a commercial fish species naturally distributed from Eastern Atlantic: British Isles to Dakar, Senegal, Canary Islands, Mediterranean and Black Sea. There is no study in our knowledge aimed to determine population genetic structuring and genetic stocks of M. barbatus species in territorial waters of Turkey. Only a few studies have been carried out on their genetics in Turkey which are limited to determination of phylogenetic relationships between species in familia of Mullidae. In this study population genetic structure and genetic diversity of red mullet (Mullus barbatus L.) in Turkish Seas was determined using sequence data of mitochondrial DNA control region.
Material and Methods: Red mullet sample were collected from the Mediterranean Sea (Mersin, Antalya), the Aegean Sea (Ayvalık), Marmara Sea (Bandırma), the Black Sea (Zonguldak, Trabzon, Fatsa and Hopa). mtDNA control region of 410 bp in length were amplified and subsequently sequenced. The sequences were aligned in Bioedit ver 7.1.3.0 (Hall, 1999). Genetic distance between populations (γst) (Nei, 1982), haplotype diversities (h), nucleotide diversities(π) were detected by DNAsp ver. 5.10 (Rozas et al., 2003). Based on pairwise distance matrix data a UPGMA dendogram was constructed by MEGA 5.05 (Kumar et al., 2004). To explain genetic structuring of samples we performed analysis of molecular variance (AMOVA) using Arlequin ver. 3.5 (Excoffier et al., 2010).
Results: In total 190 individuals were studied and alignment of partial control region of mtDNA revealed 98 mtDNA haplotypes with 75 polymorphic sites. The average of nucleotide diversities and haplotype diversities were calculated 0,015 and 0,963 respectively. Haplotype and nucleotide (π) diversities among the populations ranged from 0,907 (Zonguldak) to 0,972 (Trabzon) and from 0.0155 (Trabzon) to 0,0114 (Bandırma), respectively. Distance tree based on gammast pairwise comparisons revealed two main clades, the Aegean Sea coupled with the Mediterranean sea and the Black sea. Control region of mtDNA sequence data information of this study showed high genetic diversity and structuring among groups. (Gamma st; 0,140, Nm; 3,05). According to pairwise γSt matrix the highest genetic differentiation was found between Hopa and Ayvalık (γSt: 0.17973) and the lowest genetic differentiation was detected between Bandırma and Zonguldak (γSt: 0,01606).
Conclusions: Determination of genetic structures of fish populations and their genetic diversities are very important for perpetuation of fish populations and sustainable fisheries management. In this way, results of the present study are şmportant not only for the continuity of this species, but also would contribute to the sustainability of other fish species under the fishing pressure.
Acknowledgements
We thank to The Scientific and Technological Research Council of Turkey for financial support (TÜBİTAK, project no: 212T110)
References
Excoffier, L. ve H.E. L. 2010. Lischer Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources. 10: 564-567.
Hall, T.A., 1999. Bioedit: a user friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41.
Kumar, S., Tamura, K., Nei, M. 2004. Mega 3: integrated software for molecular evolutionary genetics analyses and sequence alignment. Briefings in Bioinformatics, 5(2):150-163.
Nei, M., 1982. Evolution of human races at the gene level. In: Bone-Tamir, B., Cohen, T., Goodman, R.M. (Eds.), Human Genetics Part A: The Unfolding Genome. Alan R Liss, New York, pp. 167–181.
Rozas, J., Sanchez-Del Barrio, J.C., Messeguer, X., Rozas, R., 2003. DnaSP, DNA polymorphizm analyses by the coalescent and other methods. Bioinformatics 19, 2496–2497.
Keywords:
Red mullet,
Mullus barbatus,
mtDNA,
Turkey,
Genetic structure,
fish
Conference:
IMMR | International Meeting on Marine Research 2014, Peniche, Portugal, 10 Jul - 11 Jul, 2014.
Presentation Type:
Poster Presentation
Topic:
FISHERIES AND MANAGEMENT
Citation:
Tuncay
D,
Senol Tuncay
S,
Yılmaz
C and
Bardakci
F
(2014). Population Genetic Structure of red mullet (Mullus barbatus L.) in Turkish Sea Based on Mitochondrial DNA.
Front. Mar. Sci.
Conference Abstract:
IMMR | International Meeting on Marine Research 2014.
doi: 10.3389/conf.fmars.2014.02.00152
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
09 May 2014;
Published Online:
18 Jul 2014.
*
Correspondence:
Prof. Fevzi Bardakci, Adnan Menderes University, Biology, Aydın, 09100, Türkiye, fevzi.bardakci@gmail.com