Effects of lipopolysaccharide preconditioning on pentylenetetrazol-induced seizure in rat model of epilepsy
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1
UKM Medical Centre (HUKM), Department of Medicine, Faculty of Medicine , Malaysia
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2
University of Malaya, Department of Pharmacology, Faculty of Medicine, Malaysia
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3
Shahid Beheshti University of Medical Sciences, Neuroscience Research Center, Iran
Epilepsy is considered as one of the oldest neurological conditions caused by repeated sudden abnormal neuronal discharges in the central nervous system (CNS). Lipopolysaccharide (LPS) preconditioning can promise to serve as an alternative therapeutic approach, because this strategy has demonstrated the capability to attenuate damage induced by seizures in rodent models. The aim of this study was to evaluate the effect of LPS preconditioning on pentylenetetrazol (PTZ)-induced seizure in rat. With respect to our hypothesis, 4 days before PTZ administration, male SD rats were induced by either low dose of LPS or normal saline as treated and control groups respectively. In the present study, the anticonvulsant effect of preconditioning by low dose of LPS against seizures elicited by PTZ was measured. Preconditioned animals performed significantly better on the behavioral tests. Furthermore, our findings were confirmed by the histological data after analyses. Our results show that LPS preconditioning reduces both behavioral symptoms and histological damage of PTZ-induced epileptic seizure in male SD rats. This report shows that the LPS preconditioning provides neuroprotection in hippocampal CA1, CA3 and DG sections which is caused by transcriptional gene reprogramming as well as neuroregeneration in the brain. Understanding the mechanisms involved in the single LPS preconditioning and the molecular pathways leading to neuroprotection may introduce an alternative therapeutic approach in the near future.
Keywords:
Epilepsy,
Hippocampus,
in vivo,
molecular mechanism,
Neurobehaviour
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
O02: Postgraduate Travel Awardees Oral Session 2
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Amini
E,
Golpich
M,
Mohamed
Z,
Azman Ali
R,
Ahmadiani
A and
Mohamed Ibrahim
N
(2016). Effects of lipopolysaccharide preconditioning on pentylenetetrazol-induced seizure in rat model of epilepsy.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00089
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Received:
04 Aug 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Dr. Elham Amini, UKM Medical Centre (HUKM), Department of Medicine, Faculty of Medicine, Kuala Lumpur, Selangore, Malaysia, inspiration.8308@gmail.com