Clitoria ternatea root extract improves cognitive impairment and cholinergic dysfunction in animal model of chronic cerebral hypoperfusion
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1
Universiti Sains Malaysia, Centre for Drug Research, Malaysia
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2
Universiti Sains Malaysia, School of Chemical Sciences, Malaysia
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3
Universiti Sains Malaysia, School of Pharmaceutical Sciences, Malaysia
Clitoria ternatea (CT) commonly known as butterfly pea has been used to promote brain function and to treat mental disorders in Indian Ayurvedic medicine. Chronic cerebral hypoperfusion (CCH) has been considered as the major cause of cognitive impairment that occurs in human aging and vascular dementia. This study aimed to investigate the effects of the methanolic extract of Clitoria ternatea root (CTR) on cognitive impairment induced by CCH in rats and to propose cholinergic system alteration as one of the mechanism of action. Male Sprague Dawley rats (200-300g) were subjected to permanent bilateral occlusion of common carotid arteries (PBOCCA) or sham-operated surgery. These rats were then given oral administration of CTR at doses of 100, 200 and 300 mg/kg, respectively for 28 days. Passive avoidance task and Morris water maze paradigms were used to evaluate the learning and memory performances. At the end of experiment, brain was removed and the acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities were measured in prefrontal cortex, hippocampus and neocortex. The methanolic extract of CTR at the dose of 200 and 300 mg/kg significantly reversed the memory impairment induced by CCH in the passive avoidance task and reduced the escape latencies during training in Morris water maze, as well as increased the percentage of time spend in the target quadrant of the maze. In addition, the increased AChE activity in prefrontal cortex and hippocampus produced by CCH was significantly inhibited by CTR at high dose. However, there was no significant changes observed in BuChE activity between groups for all regions. The results suggested that CTR could be a potential protective agent against CCH and to improve the cholinergic function which may contribute to the improving effect of CTR on learning and memory. However, further studies are needed to explore the mechanism of action of CTR.
Acknowledgements
Financial support was received from Ministry of Higher Education (MOHE) Malaysia and Universiti Sains Malaysia special funding for the project of Fundamental Neuroscience-Neurobehaviour (304/CDADAH/652201/K134) and RUI (1001/CDADAH/812177)
Keywords:
Cognition,
in vivo,
natural product,
Cholinergic function,
Behavioural test
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
Poster Presentation Session
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Damodaran
T,
Mahsufi Mansor
S,
Keat
LG,
Murugaiyah
V,
Ramanathan
S and
Hassan
Z
(2016). Clitoria ternatea root extract improves cognitive impairment and cholinergic dysfunction in animal model of chronic cerebral hypoperfusion.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00109
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Received:
04 Aug 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Dr. Zurina Hassan, Universiti Sains Malaysia, Centre for Drug Research, Georgetown, Penang, Malaysia, Zurina.Hassan@frontiersin.org