Surface refinement of thick film gold electrodes for 3D MEAs using electro polymerization of PEDOT
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1
Technische Universität Ilmenau, Electronics Technology Group, Germany
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2
Technische Universität Ilmenau, Electrochemistry and Electroplating Group, Germany
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3
Technische Universität Ilmenau, Nano-Biosystems Technology Group, Germany
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4
Technische Universität Ilmenau, Biomechatronics Group, Germany
A current challenge in biological systems modelling is transferring state-of-the-art 2-dimensional methods into the third dimensions. Beside of cell culture methods and data processing, the development of in vitro electrode arrays covering volumes of some cubic millimeters is an emerging topic for the monitoring of signal propagation in neuronal cell cultures. Mimicking real 3-dimensional tissues requires neuroelectronic hybrids which are capable to capture neuronal signals over length scales of millimeters with high accuracy. A smart assembly technology is necessary to meet all system requirements such as optimum signal transition, cell compatibility, long term stability and recycling capability of the systems. A prototype for a real 3-dimensional approach, which allows capturing of signals in a cell volume of several cubic millimeters, was recently presented. This MEA is designed to fit the MEA2100-System for in vitro recording from Multi Channel Systems. The prototype bases on Low Temperature Co-fired Ceramics, LTCC, which allow the assembly of complex 3-dimensional hybrids. The technology uses cost-effective thick film printing and allows the realization of multilayer layouts with various integrated functions. Screen printed thick film gold serves as electrode material for this first prototype of a 3 dimensional micro electrode array (MEA). The compatibility of these multilayer hybrids with directly seeded primary neurons was demonstrated. Since the properties of thick film gold electrodes can be optimized by applying different coatings, strategies were developed for the improvement of the signal transition and thus the capturing of neuronal signals. This work presents a new technology which allows direct electro polymerization of poly [3,4-ethylenedioxythiophene], PEDOT, on thick film gold electrodes using a miniaturized electrochemical cell. It comprises an Ag/AgCl reference and a platinum counter electrode, both based on thick film technology. Growth characteristics and influences on the electrochemical set-up were investigated and quantified using Design of Experiments methods. The impedance measurements, electrode topography and electrode characteristics are discussed in this paper. It is shown that the coating improves the impedance significantly when compared with bare thick film gold electrodes. The work contributes thus to an improvement of the 3D MEA technology.
Keywords:
PEDOT,
3D cell culture,
LTCC,
electrode impedance,
Electro polymerization,
thick film gold
Conference:
MEA Meeting 2016 |
10th International Meeting on Substrate-Integrated Electrode Arrays, Reutlingen, Germany, 28 Jun - 1 Jul, 2016.
Presentation Type:
Poster Presentation
Topic:
MEA Meeting 2016
Citation:
Stadie
P,
Bartsch
H,
Peipmann
R,
Ispas
A,
Baca
M,
Müller
J,
Bund
A,
Schober
A and
Witte
H
(2016). Surface refinement of thick film gold electrodes for 3D MEAs using electro polymerization of PEDOT.
Front. Neurosci.
Conference Abstract:
MEA Meeting 2016 |
10th International Meeting on Substrate-Integrated Electrode Arrays.
doi: 10.3389/conf.fnins.2016.93.00094
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Received:
22 Jun 2016;
Published Online:
24 Jun 2016.
*
Correspondence:
Dr. Hartmut Witte, Technische Universität Ilmenau, Biomechatronics Group, Illmenau, Germany, hartmut.witte@tu-ilmenau.de