Agrobacterium rhizogenes-mediated hairy root culture of Solanum mammosum (‘terung susu kambing’) for an improved solasodine production through elicitation
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1
Faculty of Dentistry, Mahsa University, Malaysia
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2
Institute of Bioscience, University Putra Malaysia, Malaysia
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3
Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia
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4
Faculty of Biotechnology and Biomolecular Sciences, Putra Malaysia University, Malaysia
Background
The aglycone of steroidal alkaloid, solasodine, which is present in Solanum mammosum, can be readily converted to 16-dehydropregnenolone, a key intermediate for the high-revenue chemical synthesis of useful steroid hormones such as progesterone and cortisone in the pharmaceutical industries. Different strains of Agrobacterium rhizogenes were used to infect S. mammosum, with the expectation to cause alteration to the phenotype and host plant secondary metabolism through the expression of root loci (rol) genes, to generate transformed hairy roots that could produce higher amount of solasodine. Two most responsive transformed hairy root lines both in the aspect of higher biomass growth and solasodine production, hairy root line-ATCC31798 and hairy root line-A4, which were separately induced by A. rhizogenes strain ATCC31798 and A4, were then used for enhancement of solasodine production through elicitation.
Methods
Different concentrations of two separate elicitors, methyl jasmonate (0 to 400 µM) and salicylic acid (0 to 200 µM), were added separately into the liquid culture medium for respective hairy root line to evaluate for the solasodine production.
Results
The addition of methyl jasmonate on day 20 of the culture gave higher growth indices with better solasodine productivity of 3.9 ± 0.4 mg/g dw and 4.1 ± 0.6 mg/g dw for hairy root line-ATCC31798 and line-A4 respectively, when compared to the other time frames tested.
Similar observation has also been observed for the additon of salicylic acid; however, the overall solasodine productivity from the two transformed hairy root lines showed 30% lesser than those obtained from methyl jasmonate treatment. Interestingly, the solasodine content obtained from the transformed hairy roots after elicitation was at least ten times higher than the untransformed roots.
Keywords:
Agrobacterium rhizogenes,
elicitor,
Hairy root,
Solanum mammosum L.,
solasodine
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Poster Presentation
Topic:
Miscellaneous
Citation:
Ooi
C,
Stanslas
J and
Maziah
M
(2019). Agrobacterium rhizogenes-mediated hairy root culture of Solanum mammosum (‘terung susu kambing’) for an improved solasodine production through elicitation.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2019.63.00034
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Received:
30 Oct 2018;
Published Online:
17 Jan 2019.
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Correspondence:
Dr. Chai Theam Ooi, Faculty of Dentistry, Mahsa University, Jenjarum, Malaysia, campbellskysky@gmail.com