Physico-chemical and biological activity of Malaysian channa striatus water extract on human foetal lung cell (IMR-90)
-
1
Universiti Kebangsaan Malaysia
, School of Chemical Sciences and Food Technology, Faculty of Science and Technology
, Malaysia
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2
Universiti Sains Malaysia, Regenerative Medicine Cluster, Advanced Medical & Dental Institute (AMDI), Malaysia
Local fishes in Malaysia including Channa species, particularly Channa striatus (haruan) have been traditionally used for wound healing and reducing pain. For that reason, this study was carried out to determine the Channa species water extract physico-chemical and its bioactivity on human foetal lung cell (IMR-90). Channa striatus (haruan) was used in this study. The sample was trimmed carefully to obtain the maximum fish fillet and extracted by using normal soaking extraction method (fish weight-to-solvent ratio = 1:4) as to procure the haruan aqueous extract. The extract was characterized for its physico-chemical properties such as pH, moisture content, rheological properties (n = 3), amino acid composition and fatty acid determination (arachidonic acid) (n = 2). The effective concentration (EC50) of the extract was conducted via 2-dimensional (2-D) culturing system on a normal human fibroblast cell (IMR90). The essential fish’s essence physical properties are as followed: 1) pH - 6.34 ± 0.01, 2) 97.3 ± 0.15% of moisture content, 3) total dissolved solid - 2.7 ± 0.01 (%, w/w) and 3) viscosity of 1.50 ± 0.31 mPa.s. The extract contained essential amino acid, glycine and fatty acid (arachidonic acid), which could aid in the process of wound healing despite their low concentration. However, the presence of those compounds have shown insignificant growth as compared to the control (without treatment) (p>0.05). Moreover, the immobile and irresponsive IMR90 growth rate on different extract concentrations was consistent with the absence of mortality throughout the 3-day incubation period (p>0.05). For that reason, the extract EC50 values could not be determined conceivably due to the high fish weight-to-solvent ratio which eventually deliver extremely low concentration of bio-active compounds for the cell-induced proliferation.
Acknowledgements
The authors would like to thank the Ministry of Higher Education (MOE) and Ministry of Science, Technology and Innovation (MOSTI), Malaysia, for providing financial support to this project (FRGS/2/2013/TK04/UKM/03/1 and 06-01-02-SF1271).
Keywords:
Wound Healing,
Aqueous extract,
Physico-chemical properties,
Channa striatus,
2-D cell culture
Conference:
6th Malaysian Tissue Engineering and Regenerative Medicine Scientific Meeting (6th MTERMS) 2016 and 2nd Malaysian Stem Cell Meeting, Seberang Jaya, Penang, Malaysia, 17 Nov - 18 Nov, 2016.
Presentation Type:
Poster
Topic:
Natural Products in Regenerative Medicine
Citation:
Shy Yi
WN,
Abdul Mutalib
SA,
Yahaya
BH and
Zubairi
S
(2016). Physico-chemical and biological activity of Malaysian channa striatus water extract on human foetal lung cell (IMR-90).
Front. Bioeng. Biotechnol.
Conference Abstract:
6th Malaysian Tissue Engineering and Regenerative Medicine Scientific Meeting (6th MTERMS) 2016 and 2nd Malaysian Stem Cell Meeting.
doi: 10.3389/conf.FBIOE.2016.02.00009
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Received:
08 Dec 2016;
Published Online:
19 Dec 2016.
*
Correspondence:
Dr. Saiful Zubairi, Universiti Kebangsaan Malaysia, School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Bangi, Selangor, 43600, Malaysia, saiful-z@ukm.edu.my