Genotyping Of Yersinia ruckeri Isolated From Rainbow Trout In Turkey By RAPD-PCR
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1
Uludag University, Department of Aquatic Animal Diseases, Türkiye
According to the UN Food and Agriculture Organization, aquaculture is growing more rapidly than all other animal food-production sectors. Particularly in Europe, the production of rainbow trout has increased more and more to supply domestic markets in the countries such as Turkey, Italy, France, Germany, Denmark, Spain etc. However, this production is hampered by unpredictable mortalities that may be due to negative interactions between fish and pathogenic bacteria like Yersinia ruckeri (Enteric Red Mouth Disease, ERM). Since ERM was first reported, knowledge of the host and geographic ranges has increased. So far, there are many reports about ERM, especially in European countries, ranging from different hosts. And now, it is one of the most important infectious diseases in rainbow trout (Oncorhynchus mykiss) aquaculture in Turkey.
In our study, Y. ruckeri isolated on diseased rainbow trout brought our laboratory between 2013 to 2016. Isolated bacteria and 5 reference strains were identified by classic microbiologic tests (Gram staining, oxidase, catalase, Oxidation/Fermentation), rapid identification kits (API RAPD ID32 E) and molecular analysis (PCR). Random Amplified Polymorphic DNA (RAPD) PCR with Enterobacterial Repetitive Intergenic Consensus-2 (ERIC) primer used for genotyping all isolates.
137 strains were separated into groups according to RAPD patterns which were 16 different genotypes (with reference strains determined 19 genotypes) with ERIC2-RAPD PCR. 117 isolates of the 137 isolates and NCTC 12266 reference strain found to be similar genetic profiles. 4 isolates of the 137 isolates and NCTC 12270 found to be similar genetic profiles. And 14 isolates of the 137 isolates were similar genetic profile within themselves. However, none of them were similar to reference strains. Strains which isolated from Aegean and Central Anatolia region were genetically similar to strains isolated in the year 2014 and 2015.
As a result, incidence of Yersiniosis in rainbow trout farms in our country is high, especially with the warming of the waters in spring. According to RAPD-PCR analysis, Y. ruckeri strains have 78.7 % similarity and other parts have 16 different genetic profile.
Keywords:
rainbow trout,
Yersinia ruckeri,
genotyping,
RAPD-PCR,
ERIC Primer
Conference:
AquaEpi I - 2016, Oslo, Norway, 20 Sep - 22 Sep, 2016.
Presentation Type:
Poster
Topic:
Aquatic Animal Epidemiology
Citation:
DUMAN
M,
ALTUN
S,
BÜYÜKEKİZ
AG and
SATICIOĞLU
IB
(2016). Genotyping Of Yersinia ruckeri Isolated From Rainbow Trout In Turkey By RAPD-PCR.
Front. Vet. Sci.
Conference Abstract:
AquaEpi I - 2016.
doi: 10.3389/conf.FVETS.2016.02.00016
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Received:
30 May 2016;
Published Online:
14 Sep 2016.
*
Correspondence:
MD, PhD. Izzet B SATICIOĞLU, Uludag University, Department of Aquatic Animal Diseases, Bursa, Türkiye, iburcinsat@uludag.edu.tr