MANGANESE NEUROTOXICITY INDUCES ATYPICAL PARKINSONISM IN THE RAT
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1
Mohammed V-Agdal University, Biology, Morocco
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2
University of Bordeaux 2, Life Sciences, France
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3
IMN UMR CNRS 5293, France
Manganese (Mn) neurotoxicity is associated with progressive disturbances in motor and cognitive functions. However, the exact mechanisms underlying these deficits remain unknown. The present study aimed to investigate the effects of Mn intoxication on: locomotor activity using an open field actimeter; motor coordination using rotarod; anxiety behavior using elevated plus maze; “depression-like” behavior using sucrose preference and forced swim test; Globus Pallidus (GP) and Subthalamic Nucleus (STN) neuronal activities by using extracellular electrophysiology. Male Sprague–Dawley rats were daily treated with MnCl2 (10mg/kg/ i.p.) for 5 weeks. Subsequently, we determined the impact of Mn on the postmortem tissue level of monoamines in different brain regions. The results show that Mn induced a gradual decrease of exploratory and locomotor activities, of motor coordination, together with anxiety and “depressive-like” behaviors. Electrophysiological results show that while majority of GP and STN neurons discharged regularly in controls, Mn induced an increase in the number of GP and STN neurons discharging irregularly and/or with bursty pattern. Moreover, Mn decreased the firing rate of GP and STN neurons. HPLC analyses show that Mn significantly decreased tissue levels of noradrenalin and serotonin in the prefrontal cortex, while an increase in the level of dopamine and its metabolite 3,4-dihydroxyphenylacetic acid was found in the striatum. This work shows for the first time that Mn resulted in motor and non motor deficits as well as changes in the activity of GP and STN neurons. These changes were paralleled by noradrenaline and serotonin depletion, in addition to an increase of dopamine metabolism. Together, these data provide evidence that Mn is responsible of pathophysiological changes similar to those observed in atypical parkinsonism.
Keywords:
Manganese,
locomotor activity,
Anxiety,
Monoamines tissue content,
Globus Pallidus,
Subthalamic Nucleus,
rat
Conference:
4th Conference of the Mediterrarnean Neuroscience Society, Istanbul, Türkiye, 30 Sep - 3 Oct, 2012.
Presentation Type:
Poster Presentation
Topic:
Abstracts
Citation:
Bouabid
S,
Delaville
C,
De Deurwaerdère
P,
Lakhdar Ghazal
N and
Benazzouz
A
(2013). MANGANESE NEUROTOXICITY INDUCES ATYPICAL PARKINSONISM IN THE RAT.
Conference Abstract:
4th Conference of the Mediterrarnean Neuroscience Society.
doi: 10.3389/conf.fnhum.2013.210.00009
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Received:
07 Feb 2013;
Published Online:
11 Apr 2013.
*
Correspondence:
Prof. Philippe De Deurwaerdère, University of Bordeaux 2, Life Sciences, Bordeaux, 33076, France, deurwaer@u-bordeaux.fr