Evaluation of the effects of cannabinoid compounds either alone or in combination with diazepam on anxiety-like behaviour in the mouse hole-board test
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1
Shaheed Beheshti University of Medical Sciences, Iran
Introduction: The effects of the cannabinoid and GABAergic compounds on anxiety-like behaviour were thoroughly investigated. However, in this study, we studied the interaction between cannabinoid and GABAergic compounds on anxiety-like behaviour of mice submitted to the hole-board model of anxiety.
Methods: The apparatus was a squared platform (30 × 30 cm) containing 25 holes equally spaced and arranged in 5 × 5 array. At the beginning of the test, each mouse was placed in the centre of the apparatus. During 5 min, the number of head dipping (i.e. when the mouse put his head into a hole) was considered as a criterion of anxiety-like behaviour.
Results: Diazepam (1 mg/kg) and cannabinoid CB1 receptor agonist, WIN55212-2 (1.2 mg/kg), significantly increased the number of head dipping compared to control group. Different doses of cannabinoid reuptake inhibitor, AM404, did not change the number of head dipping compared to control group. In addition, co-administration of AM404 and diazepam did not increase the anxiolytic effect of diazepam compared to group that received the respective dose of diazepam. Pretreatment with cannabinoid CB1 receptor antagonist, AM251, 30 min before administration of diazepam, did not change the effect of diazepam on anxiety-like behaviour of mice. Combination of sub-therapeutic doses of WIN55212-2 and diazepam did not show synergistic effect compared to single administration of respective doses of each drug.
Conclusion: Although Cannabinoids and diazepam can change the anxiety-like behaviour, but they may not have any important interaction in reducing the anxiety-like behaviour in the mouse hole-board test.
Conference:
10th International Conference on Cognitive Neuroscience, Bodrum, Türkiye, 1 Sep - 5 Sep, 2008.
Presentation Type:
Poster Presentation
Topic:
Animal Models of Cognition
Citation:
Rezaii
N,
Naderi
N,
Haghparast
A,
Alizadeh
A and
Motamedi
F
(2008). Evaluation of the effects of cannabinoid compounds either alone or in combination with diazepam on anxiety-like behaviour in the mouse hole-board test.
Conference Abstract:
10th International Conference on Cognitive Neuroscience.
doi: 10.3389/conf.neuro.09.2009.01.080
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Received:
02 Dec 2008;
Published Online:
02 Dec 2008.
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Correspondence:
Neguine Rezaii, Shaheed Beheshti University of Medical Sciences, Tehran, Iran, neguinerezaii@gmail.com