AUTHOR=Link Tobias , Seibel Christian , Voegele Ralf T. 

TITLE=Early insights into the genome sequence of Uromyces fabae

JOURNAL=Frontiers in Plant Science

VOLUME=5

YEAR=2014

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2014.00587

DOI=10.3389/fpls.2014.00587

ISSN=1664-462X

ABSTRACT=<p><italic>Uromyces fabae</italic> is a major pathogen of broad bean, <italic>Vicia faba</italic>. <italic>U. fabae</italic> has served as a model among rust fungi to elucidate the development of infection structures, expression and secretion of cell wall degrading enzymes and gene expression. Using <italic>U. fabae</italic>, enormous progress was made regarding nutrient uptake and metabolism and in the search for secreted proteins and effectors. Here, we present results from a genome survey of <italic>U. fabae</italic>. Paired end Illumina sequencing provided 53 Gb of data. An assembly gave 59,735 scaffolds with a total length of 216 Mb. K-mer analysis estimated the genome size to be 329 Mb. Of a representative set of 23,153 predicted proteins we could annotate 10,209, and predict 599 secreted proteins. Clustering of the protein set indicates families of highly likely effectors. We also found new homologs of RTP1p, a prototype rust effector. The <italic>U. fabae</italic> genome will be an important resource for comparative analyses with <italic>U. appendiculatus</italic> and <italic>P. pachyrhizi</italic> and provide information regarding the phylogenetic relationship of the genus <italic>Uromyces</italic> with respect to other rust fungi already sequenced, namely <italic>Puccinia graminis</italic> f. sp. <italic>tritici, P. striiformis</italic> f. sp. <italic>tritici, Melampsora lini</italic>, and <italic>Melampsora larici-populina</italic>.</p>