Antioxidant enzymes activities in the liver of sea bass, Dicentrarchus labrax, juveniles fed diets with different levels of vitamins
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1
Escola Superior de Turismo e Tecnologia do Mar, Politécnico de Leiria, MARE – Marine and Environmental Sciences Centre, Portugal
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2
Estação Piloto de Piscicultura de Olhão, Instituto Português do Mar e Atmosfera, Portugal
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3
Sparos (Portugal), Portugal
During the early stages of development, marine fish larvae exhibit high rates of growth and differentiation of their tissues and organs. Therefore, high metabolism and cellular differentiation is expectable, with consequent production of reactive oxygen species (ROS). These products of metabolism are known to cause oxidative stress, with detrimental effect on growth and survival of fish larvae. These organisms have effective antioxidant defences to protect cells from oxidative stress, but the efficiency of these mechanisms depends on the adequate supply of dietary antioxidants, such as vitamins. So, it is possible and evident that diet might play a protective role against ROS. The objective of this study was to assess the activity of enzymes of oxidative stress in the sea bass juveniles fed diet with different levels of vitamins.
Sea bass post-larvae were reared between 30 to 70 days according to EPPO standard rearing procedures for this species. Three experimental diets A, B and C were tested in triplicate. Diets were formulated and produced by SPAROS. Growth and survival were monitored along the trial. At the end of the trial 30 post-larvae were collected per tank. Tissues were homogenized in phosphate buffered saline solution at pH 7.4 (1g of tissue/15ml buffer), centrifuged at 13,500g, at 4ºC, for 15 min. Supernatant was divided in different aliquots and stored at -80ºC until analysis. Aliquots were used for the determination of several parameters. Oxidative damage due to lipid peroxidation was evaluated by the formation of malondialdehyde (MDA). Superoxide dismutase (SOD) activity was determined using nitroblue tetrazolium (NBT) as substrate and the reaction was monitored at 560 nm. Glutathione peroxidase (GPx) activity was assayed using cumene hydroperoxide and hydrogen peroxide as substrates for measuring total GPX and Se-dependent GPX (Se-GPX), respectively, and the reaction monitored at 340 nm. Catalase (CAT) activity was determined using hydrogen peroxide as substrate and monitoring the reaction at 240nm. The activity of enzymes was normalized by the total protein content, being expressed as U/mg of protein.
The results concerning the activity of oxidative stress enzymes of sea bass fed diets with different levels of vitamins will be presented in this study.
Acknowledgements
This study was funded by national projects DIVERSIAQUA (ref. 16-02-01-FEAM-66; MAR2020, Portugal) and LARVAMIX (ALG-01-0247-FEDER-017925; ALGARVE2020)
Keywords:
fish larvae,
Oxidative Stress,
Superoxide Dismutase,
Catalase,
Glutathione Peroxidase,
Lipid Peroxidation,
nutrition,
Premix
Conference:
IMMR'18 | International Meeting on Marine Research 2018, Peniche, Portugal, 5 Jul - 6 Jul, 2018.
Presentation Type:
Poster Presentation
Topic:
Aquaculture
Citation:
Almeida
CS,
Castanho
S,
Silva
A,
Mendes
A,
Pousão-Ferreira
P,
Viegas
M,
Dias
J,
Baptista
TM and
Ribeiro
L
(2019). Antioxidant enzymes activities in the liver of sea bass, Dicentrarchus labrax, juveniles fed diets with different levels of vitamins.
Front. Mar. Sci.
Conference Abstract:
IMMR'18 | International Meeting on Marine Research 2018.
doi: 10.3389/conf.FMARS.2018.06.00041
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Received:
02 May 2018;
Published Online:
07 Jan 2019.
*
Correspondence:
Mrs. Catarina S Almeida, Escola Superior de Turismo e Tecnologia do Mar, Politécnico de Leiria, MARE – Marine and Environmental Sciences Centre, Peniche, Portugal, Catarina.almeida75@gmail.com