Bioactivity screening of cyanobacteria for the isolation of novel anticancer compounds using 2D and 3D cell culture models
-
1
Centro Interdisciplinar de Pesquisa Marine e Ambiental (CIIMAR), Portugal
-
2
Institute of Biomedical Sciences Abel Salazar (ICBAS), Portugal
-
3
Faculdade de Ciências, Universidade do Porto, Portugal
Cyanobacteria are microorganisms who produce secondary metabolites potentially bioactive against multiple activities, such as anticancer. Considered the second biggest cause of world mortality, treatment of cancer aims for more and more diverse therapeutics. Hence, the usage of these organisms is of major interest for marine biotechnology, as a source of new natural drugs.
This work focusses on the isolation of natural compounds from cyanobacteria with potential anticancer activity. For that, we followed a bioassay-guided fractionation of cyanobacteria biomass extracts, from multiple strains of the LEGE Cyanobacteria Culture Collection. The obtained crude extract was fractionated through vacuum liquid chromatography, resulting in fractions from less to more polar, who were tested on 2D cell culture of MG-63 cell line through MTT assays and on 3D cell culture of HCT-116 cell line using fluorescence microscopy. The 3D cell model mimics solid tumour in vitro and therefore is a physiologically more relevant model than monolayer cultures.
Our results showed that different fractions have activity in 2D than in 3D cultures, unveiling selectivity for cell lines and possible differences in chemical properties of bioactive compounds. We will continue the fractionation of biologically active fractions, through column chromatography and HPLC, to obtain an isolated compound and to elucidate its chemical structure using methods such as LC-MS and NMR spectroscopy, proving the interest of these microorganisms as a potential source of new drugs.
Acknowledgements
Acknowledgement: This work was supported by the project INNOVMAR - Innovation and Sustainability in the Management and Exploitation of Marine Resources (reference NORTE-01-0145-FEDER-000035, within the research line NOVELMAR, supported by North Portugal Regional Operational Program (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).
Keywords:
Cyanobacteria metabolites,
Bioassay guided fractionation,
Anticancer activity,
2D cell models,
3D cell models,
MTT assay,
fluorescence microscopy,
NMR spectroscopy,
LC-MS
Conference:
IMMR'18 | International Meeting on Marine Research 2018, Peniche, Portugal, 5 Jul - 6 Jul, 2018.
Presentation Type:
Poster Presentation
Topic:
Blue Biotech
Citation:
Ferreira
L,
Sousa
ML,
Preto
M,
Vasconcelos
V and
Urbatzka
R
(2019). Bioactivity screening of cyanobacteria for the isolation of novel anticancer compounds using 2D and 3D cell culture models.
Front. Mar. Sci.
Conference Abstract:
IMMR'18 | International Meeting on Marine Research 2018.
doi: 10.3389/conf.FMARS.2018.06.00089
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
29 Apr 2018;
Published Online:
07 Jan 2019.
*
Correspondence:
Miss. Leonor Ferreira, Centro Interdisciplinar de Pesquisa Marine e Ambiental (CIIMAR), Matosinhos, Portugal, leonor.amaral.s.f@gmail.com
Dr. Ralph Urbatzka, Centro Interdisciplinar de Pesquisa Marine e Ambiental (CIIMAR), Matosinhos, Portugal, rurbatzka@ciimar.up.pt