Development of custom assays for the autonomous detection and quantification of human enteric viruses in seawater using Lab on a Chip
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1
University of Southampton, United Kingdom
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2
National Oceanography Centre Southampton, Ocean Technology and Engineering, United Kingdom
Epidemiological evidence identifies human enteric viruses, such as Hepatitis A and Norovirus, as the most common pathogens transmitted by bivalve shellfish. Of these Hepatitis A is the most serious viral infection causing a debilitating disease and death in severe cases. Molecular methods have become an important tool in marine biological sciences because they can identify genetically distinct entities and active biological processes. Many of these techniques are amenable to miniaturisation and miniaturised sensors have great potential in environmental and ecological monitoring, particularly for harmful microorganisms, many of which are unculturable. Several challenges remain however with regards to developing these systems for deployment in the field. We address these challenges for a novel Lab on a Chip sensor prototype, the Isotherminator, which supports nucleic acid amplification based assays for bacterial and viral pathogens. We present here different methodologies for the concentration and extraction of RNA viruses from seawater, and the results of on-chip nucleic acid amplification of Hepatitis A, Norovirus, and Hepatitis E, demonstrating nucleic acid quantification on targeted genes is possible using pre-manufactured chips with custom reagents combined with LOC analytics.
Keywords:
Hepatitis A,
Norovirus,
NASBA,
Isothermal amplification,
lab-on-a-chip
Conference:
IMMR'18 | International Meeting on Marine Research 2018, Peniche, Portugal, 5 Jul - 6 Jul, 2018.
Presentation Type:
Poster Presentation
Topic:
Blue Biotech
Citation:
Wilson
M,
Robidart
J,
Balan
C,
Cardwell
C,
Mowlem
MC and
McQuillan
J
(2019). Development of custom assays for the autonomous detection and quantification of human enteric viruses in seawater using Lab on a Chip.
Front. Mar. Sci.
Conference Abstract:
IMMR'18 | International Meeting on Marine Research 2018.
doi: 10.3389/conf.FMARS.2018.06.00120
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Received:
19 Apr 2018;
Published Online:
07 Jan 2019.
*
Correspondence:
Mr. Matthew Wilson, University of Southampton, Southampton, United Kingdom, mw6g14@soton.ac.uk