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SHOTGUN ANALYSIS TO LOOK FOR DIFFERENCES IN IMMUNE ABILITY BETWEEN GRANULOCYTES AND HYALINOCYTES OF THE EUROPEAN FLAT OYSTER

Nuria R. De La Ballina1*, Antonio Villalba1 and Asuncion Cao1
  • 1 Centro de Investigacións Mariñas (CIMA), Pathology, Spain

Recovery and maintenance of wild populations of the European flat oyster Ostrea edulis should be recognized as a priority for habitat management and conservation of this species, which is close to functional extinction throughout European coasts. Diseases, particular bonamiosis, have contributed to oyster decline and are important constrains for oyster recovery. Understanding oyster immune system should contribute to design effective strategies to fight oyster diseases. Haemocytes play a pivotal role in mollusc immune responses protecting from infection. Two main types of haemocytes, granulocytes and hyalinocytes, are distinguished in O. edulis. Even though both cell types participate in immune responses, granulocytes are thought to be the main immunocompetent cells as they show higher phagocytic ability, while knowledge on specific immune functions of hyalinocytes is scarce. The current study aims at enriching the current knowledge regarding Ostrea edulis innate immunity by comparing the proteome of the two haemolymph cell types. We performed shotgun proteomics, which allows identifying a high number of proteins, thus providing broad view of cell function and physiology. Cells from oyster haemolymph were differentially separated by Percoll density gradient centrifugation. Shotgun performance through LC MS/MS allowed the identification of 218 proteins in hyalinocytes and 212 in the proteome of granulocytes. After a comparative study 112 proteins were found as exclusive of hyalinocytes from which 17 are involved in immune responses. In granulocytes 114 exclusive proteins were detected from which 24 proteins highlight because its main role in defense. Our research contributes to evaluate functional differences between granulocytes and hyalinocytes and to better understand the oyster immunity mechanisms, which could have direct implications in the organism integrity preservation.

Acknowledgements

This study was funded by the Consellería do Mar da Xunta de Galicia and the OYSTERECOVER project (FP7-SM3-2008-2-243583) within the 7th Framework Programme of the European Union.

References


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Keywords: Ostrea edulis, Haemolymph, Proteomics, LC MS/MS, immunology

Conference: IMMR'18 | International Meeting on Marine Research 2018, Peniche, Portugal, 5 Jul - 6 Jul, 2018.

Presentation Type: Oral Presentation

Topic: Biodiversity, Conservation and Coastal Management

Citation: De La Ballina NR, Villalba A and Cao A (2019). SHOTGUN ANALYSIS TO LOOK FOR DIFFERENCES IN IMMUNE ABILITY BETWEEN GRANULOCYTES AND HYALINOCYTES OF THE EUROPEAN FLAT OYSTER. Front. Mar. Sci. Conference Abstract: IMMR'18 | International Meeting on Marine Research 2018. doi: 10.3389/conf.FMARS.2018.06.00125

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Received: 31 Mar 2018; Published Online: 07 Jan 2019.

* Correspondence: Ms. Nuria R De La Ballina, Centro de Investigacións Mariñas (CIMA), Pathology, Vilanova de Arousa, Pontevedra, 36620, Spain, ondanua@gmail.com