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Catostylus tagi: in vitro fertilization and cell cleavage

Raquel Lisboa1, 2*, Paulo Mascarenhas2, Fátma Gil3, Zilda B. Morais2 and Andre C. Morandini4, 5
  • 1 Departamento de Biologia, Universidade de Aveiro, Portugal
  • 2 Instituto Superior de Ciências da Saúde Egas Moniz, Portugal
  • 3 Aquário Vasco da Gama, Portugal
  • 4 Instituto de Biociências, Universidade de São Paulo, Departamento de Zoologia, Brazil
  • 5 Universidade de São Paulo, Centro de Biologia Marinha, Brazil

In terms of vulnerability to climate change effects, invertebrates and fish are most affected with consequences on the productivity and distribution of their populations [1]. The long-term survival of their populations depend on the development of benthic stages, which have gained considerable attention in recent years due to their impact on human activities and ecosystems [2]. Catostylus tagi is an edible scyphomedusa (but still without commercial exploitation) which occurs in summer at Tagus and Sado estuaries. In view of its application in health sciences, some studies have already been started [3]. In September 2017, twenty specimens were randomly collected at Tagus estuary and the gonads were removed and brought to laboratory. In the Egas Moniz laboratory, samples of the gonads were removed from each individual, the sex was identified under the microscope, and gastric filaments were removed. To initiate the in vitro fertilization samples of different males and females were placed in containers with 80 mL of Tagus water and most of them with substrate. The process of development from the fertilized egg to a polyp with 4 tentacles in advanced state took a total of 72 hours. It was observed that from the fertilized egg to the beginning of cell division only 6 hours passed and the formation of ~ 64 cells was completed in 15 hours. After 24 hours of fertilization, planulae were formed and developed completely in a newly settled polyp in 2 hours. On the first day of settlement, 257 organisms were obtained, 176 of which were planulae and 81 polyps. Other authors have observed a similar process of in vitro fertilization for other jellyfish species, however slightly longer [4] [5]. The effectiveness and speed of fertilization of C. tagi may be due to the presence of favourable conditions throughout the process (temperature, salinity and gentle movement).

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Acknowledgements

This study was funded by Egas Moniz Coop Ensino Superior CRL, project EM-ZM 03/04.

References

[1] Hare, J. A., Morrison, W. E., Nelson, M. W., Stachura, M. M., Teeters, E. J., Griffis, R. B., ... & Chute, A. S. (2016). A vulnerability assessment of fish and invertebrates to climate change on the Northeast US Continental Shelf. PloS one, 11(2), e0146756.
[2] Helm, R. R. (2018). Evolution and development of scyphozoan jellyfish. Biological Reviews, 93, 1228–1250.
[3] Morais, Z., Schiariti, A. & Morandini, A. (2017). An interdisciplinary approach to the scyphozoans of the Atlantic Ocean. Chapter 5 in Jellyfish: Ecology, Distribution Patterns and Human Interactions. Editor Mariottini, G.L., Nova Science Publishers, Incorporated, New York.
[4] Ohtsu, K., Kawahara, M., Ikeda, H., & Uye, S. I. (2007). Experimental induction of gonadal maturation and spawning in the giant jellyfish Nemopilema nomurai (Scyphozoa: Rhizostomeae). Marine Biology, 152(3), 667-676.
[5] Schiariti, A., Kawahara, M., Uye, S., & Mianzan, H. W. (2008). Life cycle of the jellyfish Lychnorhiza lucerna (Scyphozoa: Rhizostomeae). Marine Biology, 156(1), 1

Keywords: Catostylus tagi, Tagus river, in vitro fertilization, Planulae, Polyps

Conference: IMMR'18 | International Meeting on Marine Research 2018, Peniche, Portugal, 5 Jul - 6 Jul, 2018.

Presentation Type: Poster Presentation

Topic: Aquaculture

Citation: Lisboa R, Mascarenhas P, Gil F, Morais ZB and Morandini AC (2019). Catostylus tagi: in vitro fertilization and cell cleavage. Front. Mar. Sci. Conference Abstract: IMMR'18 | International Meeting on Marine Research 2018. doi: 10.3389/conf.FMARS.2018.06.00131

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Received: 28 Apr 2018; Published Online: 07 Jan 2019.

* Correspondence: Miss. Raquel Lisboa, Departamento de Biologia, Universidade de Aveiro, Aveiro, Portugal, raquelreislisboa@gmail.com