Evaluation of the antimicrobial potential of sulfated polysaccharides from the marine invasive algae Asparagopsis armata (Harvey, 1855).
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1
MARE – Marine and Environmental Sciences Centre, ESTM, Polytechnic Institute of Leiria, Portugal
The marine environment harbors a high number of ecosystems, where a wide diversity of organisms capable of producing interesting secondary metabolites can be found. Among these organisms are the macroalgae, sources of renewable resources characterized as being rich in biological properties [1]. Among other metabolites, these present sulfated polysaccharides (SPs) in the constitution of their cell wall, molecules widely studied for their biotechnological potential. Asparagopsis armata (Harvey, 1855) is an invading seaweed native to the Australian coast that has been spreading throughout the Portuguese coast [2]. The invasive status of this algae and low economical value, potentiate the search for novel compelling applications, valorizing this specie. Accordingly, this study evaluates the antimicrobial properties of SPs, extracted from Asparagopsis armata collected in the Peniche coast, against Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), Candida albicans (ATCC 10231), Pseudomonas aeruginosa (ATCC 27853) and Salmonella enterica (ATCC 13076) through growth inhibition analysis. The growth of E. coli, S. aureus, P. aeruginosa and S. enterica was performed in LB medium while the growth of C. albicans was performed in yeast-extract-peptone-dextrose, placed in a shaker incubator at a temperature of 37 °C until reach exponential growth (OD600). For the evaluation of the inhibitory capacity, the microorganisms were subjected to SP samples at a concentration range of 10 – 500 µg mL-1. The inhibitory effects were most expressive against S. aureus and P. aeruginosa, with an IC50 of 31.51 (25.29-39.26) µg mL-1 and 309.90 (248.00 – 384.90) µg mL-1, respectively. All statistical analysis was performed using GraphPad Prism (Version 7.04) software for windows. Our results demonstrate that Asparagopsis armata presents a potential applicability as antibacterial against S. aureus and P. aeruginosa, thus being able to grant some economic value from this invasive seaweed.
Acknowledgements
Authors would like to acknowledge the financial support of the Portuguese Foundation for Science and Technology (FCT) through Strategic Project UID/MAR/04292/2013 granted to MARE – Marine and Environmental Sciences Centre, through Red2Discovery Project (PTDC/MAR-BIO/6149/2014), co-financed by COMPETE (POCI-01-0145-FEDER-016791), and through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015 - LISBOA-01-0145-FEDER-016405).
References
1. Abu-Ghannam, N., & Rajauria, G. (2013). Antimicrobial activity of compounds isolated from algae. In Functional Ingredients from Algae for Foods and Nutraceuticals (pp. 287–306). Elsevier. https://doi.org/10.1533/9780857098689.2.287
2. Susete, P., Agnieszka, R., Joana, S., Christien, L., Marco, L., & Rui, P. (2016). The marine invasive Asparagopsis armata (Harvey, 1855) as source of bioactive valuable compounds - Antioxidant potential enrichment by Vacuum liquid Chromatography. Frontiers in Marine Science, 3. https://doi.org/10.3389/conf.FMARS.2016.04.00067
Keywords:
seaweeds,
bioactive compounds,
Broth microdilution method,
Staphylococcus aureus,
Pseudomonas aeruginosa
Conference:
IMMR'18 | International Meeting on Marine Research 2018, Peniche, Portugal, 5 Jul - 6 Jul, 2018.
Presentation Type:
Poster Presentation
Topic:
Blue Biotech
Citation:
Teodoro
F,
Gomes
F,
Pinteus
S,
Alves
C,
Silva
J,
Bernardino
SM and
Pedrosa
RP
(2019). Evaluation of the antimicrobial potential of sulfated polysaccharides from the marine invasive algae Asparagopsis armata (Harvey, 1855)..
Front. Mar. Sci.
Conference Abstract:
IMMR'18 | International Meeting on Marine Research 2018.
doi: 10.3389/conf.fmars.2018.06.00161
Copyright:
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Received:
27 Jul 2018;
Published Online:
07 Jan 2019.
*
Correspondence:
PhD. Rui P Pedrosa, MARE – Marine and Environmental Sciences Centre, ESTM, Polytechnic Institute of Leiria, Peniche, Portugal, rpedrosa@ipleiria.pt