Electrophysiological properties of primary culture neurons from mice cortex
-
1
Royan Institute, Stem cell and Development, Iran
Primary culture is a valuable model for several types of researches. For instance, drug screening, exploring the effects of stress on neural cells like hypoxia and also it can be used as a positive control for in vitro differentiated neurons. Accordingly, determination of electrophysiological properties of primary culture neurons give us the idea of native neuron’s features. Primary culture neurons can be isolated from various parts of a rodent brain like cortex, hippocampus and cerebellum. In this study, we have isolated cortical neurons from E16 embryo’s brain of NMRI mice. Then, after 12 days in vitro we have explore electrophysiological properties of these neurons by whole cell patch clamp recording technique for determining their functional properties as a positive control for differentiated neurons.
Keywords:
primary culture,
Electrophysiology,
patch clamp,
cortical neurons,
Action Potentials,
synaptic current
Conference:
12th National Congress of the Belgian Society for Neuroscience, Gent, Belgium, 22 May - 22 May, 2017.
Presentation Type:
Poster Presentation
Topic:
Development
Citation:
Mirsadeghi
S and
Kiani
S
(2019). Electrophysiological properties of primary culture neurons from mice cortex.
Front. Neurosci.
Conference Abstract:
12th National Congress of the Belgian Society for Neuroscience.
doi: 10.3389/conf.fnins.2017.94.00086
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
12 Apr 2017;
Published Online:
25 Jan 2019.
*
Correspondence:
Dr. Sahar Kiani, Royan Institute, Stem cell and Development, Tehran, 19395-4644, Iran, skiani2536@gmail.com