Choroid-plexus derived extracellular vesicles in Alzheimer’s disease and Parkinson’s disease: spreading the word
Charysse
Vandendriessche1, 2*,
Sriram
Balusu3,
Arnout
Bruggeman1, 2, 4,
Caroline
Van Cauwenberghe1, 2,
Griet
Van Imschoot1, 2,
Elien
Van Wonterghem1, 2 and
Roosmarijn
Vandenbroucke1, 2
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1
VIB-UGent Center for Inflammation Research, Belgium
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2
Department of Biomedical Molecular Biology, Ghent University, Belgium
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3
VIB & KU Leuven Center for Brain & Disease Research, Belgium
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4
Department of Neurology, Ghent University Hospital, Belgium
Objectives: The blood-cerebrospinal fluid barrier is formed by choroid plexus epithelial (CPE) cells, a single layer of epithelial cells situated at the interface between blood and the cerebrospinal fluid (CSF-) containing ventricular cavities. We could show that the CPE cells secrete more extracellular vesicles (EVs) into the CSF in the context of systemic inflammation (Balusu et al., EMBO Mol Med, 2016). We are currently studying this process in the context of Alzheimer’s disease (AD) and Parkinson’s disease (PD).
Methods: We mimic AD or PD by the intracerebroventricular (icv) injection of respectively amyloid beta oligomers (AβO) or recombinant human alpha synuclein sonicated pre-formed fibrils (αSyn sPFFs) in wild type mice. Quantification of the amount of vesicles in the CSF is done using Nanoparticle Tracking Analysis (NTA) and the importance of the CPE cells as the source of EVs is studied using immunostainings, transmission electron microscopy and primary CPE cultures.
Results: We found that after the icv injection of AβO or αSyn sPFFs, significantly more EVs are present in the CSF. We could show that at least an important part of these EVs are CPE-derived. Moreover, our models are associated with brain inflammation and the occurrence of behavioural deficits. Interestingly, we have indications that the CPE-derived EVs might play a role in these events.
Conclusion: Our data show that AβO and αSyn sPFFs induce EV secretion into the CSF. Further research is needed to determine what their exact role in the pathogenesis of respectively AD and PD might be.
Keywords:
Choroid plexus (CP),
Extracellular vesicle (EV),
Alzheimer's disease (AD),
Parkinson's disease (PD),
Blood-cerebrospinal fluid barrier (BCSFB)
Conference:
13th National Congress of the Belgian Society for Neuroscience , Brussels, Belgium, 24 May - 24 May, 2019.
Presentation Type:
Poster presentation
Topic:
Cellular/Molecular Neuroscience
Citation:
Vandendriessche
C,
Balusu
S,
Bruggeman
A,
Van Cauwenberghe
C,
Van Imschoot
G,
Van Wonterghem
E and
Vandenbroucke
R
(2019). Choroid-plexus derived extracellular vesicles in Alzheimer’s disease and Parkinson’s disease: spreading the word.
Front. Neurosci.
Conference Abstract:
13th National Congress of the Belgian Society for Neuroscience .
doi: 10.3389/conf.fnins.2019.96.00008
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Received:
30 Apr 2019;
Published Online:
27 Sep 2019.
*
Correspondence:
Mx. Charysse Vandendriessche, VIB-UGent Center for Inflammation Research, Ghent, Belgium, charyssevdd@irc.vib-ugent.be