Transcriptional gene expression profiles on nasopharyngeal carcinoma/HK1 cell line with siRac1-mediated knockdown
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1
Cluster of Oncological and Radiological Science, Advanced Medical and Dental Institute, University of Science, Malaysia, Malaysia
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2
Department of Medical Laboratory Science, Faculty of Health Science and Technology, Ebonyi State University, Nigeria
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3
Regenerative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Malaysia
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4
Institute for Research in Molecular Medicine, University of Science Malaysia, Malaysia
Background
In Asia, nasopharyngeal carcinoma (NPC) is one of the leading head and neck cancer which is often diagnosed at late stage with poor prognosis. The critical regulatory pathways involved in NPC malignancy, resistance behavior and recurrence cases are still not clear. Rac1 has become a promising therapeutic target for cancer associated with reactive oxygen species-mediated cell killing.
Methods
This work profiled a set of genes associated in NPC malignancy phenotype via siRac1 (Small interfering RNA for Rac1 gene) activities. HK1 cell line was given by Professor George Tsao from University of Hong Kong represents the differentiated squamous nasopharynx carcinoma.
Results
Findings from RT-qPCR results revealed initial gene regulations kindred in cell cycle and apoptotic regulators such as CDC42, CDH1, BAX, MAPK14 and PIK3CA. Further significant regulation of genes such as AKT1, TGF-β1, BCL2 and KRAS was observed that could be involved in suppressing the NPC malignancy phenotype.
Conclusion
This study suggests that NPC/HK1 cells with siRac1-mediated knockdown might be involved in anti-metastatic role and pro-apoptotic pathways. Further understanding on these mechanisms are crucial in identifying potential NPC therapeutic targets.
Acknowledgements
The authors would like to acknowledge Bridging Research Grant: 304/CIPPT/6316184 from Universiti Sains Malaysia for sponsoring this work
Keywords:
siRNA - small interfering RNA,
RNAi therapeutics,
siRac1,
Reactive oxygen Species-Mediated Cell Killing,
Nasopharyngeal carcinoma (NPC)
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Oral Presentation
Topic:
Cancer
Citation:
S. M. N. Mydin
R,
Okekpa
SI,
Moses
EJ,
Azlan
A,
Kaur
G and
Musa
M
(2019). Transcriptional gene expression profiles on nasopharyngeal carcinoma/HK1 cell line with siRac1-mediated knockdown.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00026
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Received:
23 Oct 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Rabiatul Basria S. M. N. Mydin, Cluster of Oncological and Radiological Science, Advanced Medical and Dental Institute, University of Science, Malaysia, Pulau Pinang, Malaysia, rabiatulbasria@usm.my