Chemopreventive and antioxidant potential effects of oleuropein on two-stage skin carcinogenesis mouse model
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1
Centre of Health & Applied Sciences, Faculty of Health Sciences, National University of Malaysia, Malaysia
Background
Oleuropein is a phenylethanoid, a form of a phenolic compound which found in olive leaf. It is known to have numerous pharmacological roles such as antioxidant, antibacterial, anti-inflammatory and anti-tumour. This study was carried out to investigate the effect of oleuropein on the two-stage skin carcinogenesis mouse model including the initiation and promotion stages.
Methods
Skin tumours were initiated by topical application of 7,12-dimethylbenz[a]anthracene (DMBA: 200nmol) and promoted by 12-O-tetradecanoylphorbol-13-acetate (TPA: 20nmol) on the shaved dorsal area of mice. Total of 30 female ICR mice was randomly divided into five groups (n=6 per group). Group I was treated with DMBA and TPA, group II as a negative control was treated with acetone (70%) alone, group III to V are treatment groups (10mg/kg): oleuropein pre-initiated group (Group III), oleuropein post-initiated group (Group IV) and oleuropein pre- and post-initiated group (Group V).
Results
After 16 weeks, group III showed a significant reduction in the percentage of mouse bearing tumour (p<0.05) and the mean number of tumour per mouse (p<0.05) as compared to group I. Based on histopathological analysis, group III prevented skin carcinogenesis with mild epidermal hyperplasia formation even after 16 weeks of DMBA/TPA induction. Moreover, the level of lipid peroxidation (MDA) was significantly reduced in group III compared to group I (p<0.05). The level of antioxidant superoxide dismutase (SOD) was significantly increased in group III (p<0.05), while the level of glutathione (GSH) was significantly higher in Group V (p<0.05) compared to group I.
Conclusion
These findings indicate that oleuropein may act as a potent chemopreventive agent at the pre-initiation stage against the development of mouse skin carcinogenesis through its antioxidant actions.
Acknowledgements
This work was financially funded by the National University of Malaysia, research grant scheme (GGPM-2016-060). The authors also would like to thank all the lecturers and staff of the Biomedical Science Programme, Faculty of Health Sciences, Universiti Kebangsaan Malaysia.
Keywords:
oleuropein,
Skin Cancer,
antioxidant,
Chemoprevention,
Carcinogenesis
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Oral Presentation
Topic:
Cancer
Citation:
John
DN,
Surien
O,
Abdul Hamid
Z and
Masre
SF
(2019). Chemopreventive and antioxidant potential effects of oleuropein on two-stage skin carcinogenesis mouse model.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00027
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Received:
30 Sep 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Siti F Masre, Centre of Health & Applied Sciences, Faculty of Health Sciences, National University of Malaysia, Kuala Lumpur, Malaysia, sitifathiah@ukm.edu.my