In vivo topical delivery of ethosomal formulation composed of Orthosiphon stamineus extract complexed with sophorolipid against melanoma
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1
School of Pharmaceutical Sciences, University of Science Malaysia, Malaysia
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2
Emanbiodiscoveries.sdn.bhd., Malaysia
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3
University of Science, Malaysia, Malaysia
Background
Melanoma is one of the most aggressive types of skin cancers. Anti-angiogenic compounds can suppress melanoma cells. Orthosiphon stamineus (O.S) is a well-known herb with anticancer and anti-angiogenic effects. The health benefits of O.S extract is mainly due to its high antioxidant activity that can be attributed to rosmarinic acid (RA). However, RA has a limited ability to penetrate through the skin because of its hydrophilicity via topical application. The aim of this study is therefore to improve the penetrability of O.S extract through skin by sophorolipid (SL) nanovesicles, namely ethosome in mice.
Methods
Ethosome was made using thin film hydration method with sophorolipid as the surfactant. The ethosomal formulation of O.S (O.S-SL) was prepared at different concentrations and were physico-chemically characterized. Anti-angiogenic studies were then conducted. Anti-melanoma study was performed in albino mice using melanoma cell line (B16F10).
Results
The optimized vesicle was 387nm in size with -35.6mV potential and exhibited 10 times better permeation ability when compared to the hydroethanolic solution of O.S extract. O.S-SL revealed 100% anti-angiogenic effect ex vivo and 95.8% anti-melanoma effect in vivo.
Conclusion
The results of this study demonstrated that O.S-SL can be absorbed efficiently via topical application to the deeper layers of the skin. The formulation has shown promising anti-angiogenic and anti-melanoma effects after topical applications.
Acknowledgements
The authors would like to express their gratitude to Universiti Sains Malaysia, EMAN Biodiscoveries Sdn. Bhd. and NatureCeuticals Sdn. Bhd. for providing research facilities.
Keywords:
Orthosiphon stamineus,
Ethosome,
Sophorolipid,
Melanoma,
Topical
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Oral Presentation
Topic:
Cancer
Citation:
Nazari Vishkaei
M,
Khadeer Ahamed Basheer
M and
Malik Shah Abdul Majid
A
(2019). In vivo topical delivery of ethosomal formulation composed of Orthosiphon stamineus extract complexed with sophorolipid against melanoma.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00034
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Received:
21 Oct 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Prof. Amin Malik Shah Abdul Majid, University of Science, Malaysia, George Town, Penang, 11800, Malaysia, aminmalikshah@gmail.com