Aqueous extract of Garcinia atroviridis fruit improves oxidative status of high fat diet-induced hyperlipidemic rats
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1
Department of Pharmacology, School of Pharmaceutical Sciences, University of Science Malaysia, 11800, Malaysia
Background
Garcinia atroviridis, is a well-known plant in South East Asia, commonly used in cooking and traditional medicine. The present study was undertaken to evaluate the effect of aqueous extract of ripe fruits (AqE-RFS) on the oxidative status of high-fat diet (HFD) induced hyperlipidemic rats.
Methods
Male Sprague-Dawley (SD) rats are divided into six groups of 6 rats per group. Control group rats received normal control diet for a period of six weeks. The rest of animals were fed with HFD and simultaneously treated orally with different doses of AqE-RFS (250, 500 and 1000mg/kg body weight/day).
Results
Oral administration of the AqE-RFS for six weeks significantly reduced the liver and serum lipid peroxidation by decreasing malondialdehyde (MDA) levels at both doses of 500mg/kg (p<0.05) and 1000mg/kg (p<0.01) when compared to HFD control rats. The levels of hepatic glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) were significantly (p<0.01) increased in animals treated with AqE-RFS at 1000mg/kg. Similarly, in serum of HFD treated rats with 1000mg/kg, there was a significant increase (p<0.001) in GSH and SOD levels as well as significant in CAT, GPx and GR levels (p<0.01) as compared to the HFD rats. Strong negative correlations were observed between hepatic and serum MDA contents and antioxidant markers.
Conclusion
Administration of AqE-RFS attenuates the oxidative stress and increased the antioxidant enzymes in a dose-dependent manner. The findings revealed that AqE-RFS has potential protective effect against oxidative damage in HFD-induced hyperlipidemic rats.
Acknowledgements
The authors would like to thank Universiti Sains Malaysia (USM) and the Ministry of Higher Education, Malaysia for providing financial support and research facilities. This project was funded by the Fundamental Research Grant Scheme from the Ministry of Higher Education, Malaysia (Grant number: 203/PFarmasi/6711190), and Postgraduate Research Grant Scheme from the Institute of Postgraduate Studies (IPS), USM (Grant number: 1001/PFarmasi/846071).
Keywords:
Garcinia atroviridis,
high-fat diet,
Hyperlipidemia,
Lipid Peroxidation,
antioxidant
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Oral Presentation
Topic:
Metabolic diseases
Citation:
Al-Mansoub
MA,
Asmawi
MZ and
Murugaiyah
V
(2019). Aqueous extract of Garcinia atroviridis fruit improves oxidative status of high fat diet-induced hyperlipidemic rats.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00064
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Received:
16 Oct 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Majed A Al-Mansoub, Department of Pharmacology, School of Pharmaceutical Sciences, University of Science Malaysia, 11800, Penang, 11800, Malaysia, madjed_25@yahoo.fr
Dr. Vikneswaran Murugaiyah, Department of Pharmacology, School of Pharmaceutical Sciences, University of Science Malaysia, 11800, Penang, 11800, Malaysia, vicky@usm.my