Analytical and Biological Studies of Seed Extracts of Daucus carota L. (Black Carrot)
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1
University of the Punjab, Pakistan
Background:
Natural medicinal plants are considered an important approach in search of new therapeutic agents. Even today they were used for the treatment of numerous ailments due to their cost-effective properties, less side effects and high efficacy. Daucus carota L. subsp. sativus (Hoffm.) Arcang. var. vavilovii Mazk. (Family: Apiaceae), is locally known as Kali gajar in Pakistan. Literature has shown that therapeutically important phytochemicals present which strengthen it a suitable candidate for further research. The present study aimed to standardize the seed powder, extracts and then evaluate for in vitro antioxidant, in vitro and in vivo anti-inflammatory activities.
Methodology:
n-Hexane, chloroform and methanol extracts were prepared by hot extraction method. The extracts were screened for their antioxidant potential using different in vitro models such as DPPH, FRAP, H2O2, phosphomolybdenum assay. The in vitro anti-inflammatory activity was carried out by inhibition of protein denaturation method taking diclofenac sodium as standard. The in vivo anti-inflammatory activity was executed using carrageenan induced rat paw edema taking aspirin as standard.
Result:
The proximate analysis revealed that total ash, moisture content present within the stated limits, higher amount of fats (49.1 ± 0.23) than proteins (13.3 ± 0.30) and carbohydrates (31.917 ± 0.50. The minerals detected in mg/g were iron (293), manganese (80 ), zinc (59.5), copper (23.5), nickel (20), lead (6.0) and chromium (5.0). The antioxidant studies revealed that all extracts had significant antioxidant potential but methanol extract exhibited the highest percentage. The antioxidant activity (%) of methanol extract (DPPH, 97; FRAP, 95; H2O2, 89; phosphomolybdenum assay, 67). Anti- inflammatory analysis showed that at higher doses (200 and 400 mg/kg) methanol extract had significant anti-inflammatory activity.
Conclusion:
The ability of the extract to scavenge different free radicals indicated that it may be a useful therapeutic agent for treating radical related pathological damage as in rheumatoid arthritis, acute and chronic neurodegenerative ailments, diabetes and inflammatory bowel disease in future.
Acknowledgements
A ll authors would like to acknowledge University College of pharmacy , University of the Punjab, Lahore, Pakistan.
Keywords:
Daucus carota L., seeds,,
DPPH,
antioxidant activity,,
anti-inflammatory activity,
Free Radicals
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Poster Presentation
Topic:
Miscellaneous
Citation:
Latif
A,
Nabi
MG,
Anwar
R and
Maab
H
(2019). Analytical and Biological Studies of Seed Extracts of Daucus carota L. (Black Carrot).
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00079
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Received:
27 Sep 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Prof. Abida Latif, University of the Punjab, Lahore, Pakistan, abidalatif43@yahoo.com