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Lignosus rhinocerotis water-soluble sclerotial extract relaxes rat isolated aortae.

Kayatri Govindaraju1, Mei Kee Lee1, Kang Nee Ting1*, Chon Seng Tan2, Szu Ting Ng2, Hwei-San Loh1, Sue Mian Then1 and Suresh K. Mohankumar1
  • 1 University of Nottingham Malaysia Campus, Department of Biomedical Sciences, University of Nottingham Malaysia Campus, 43500 Semenyih, Malaysia, Malaysia
  • 2 LiGNO Biotech Sdn Bhd, Malaysia

Background Lignosus rhinocerotis (Cooke) Ryvarden commonly known as Tiger Milk Mushroom is a popular traditional medicinal mushroom used in Southeast Asia to treat many conditions including asthma, cough and cancer. Many other edible mushrooms such as Cordycep sinensis have reported to exhibit antihypertensive effects. The study aims to investigate the vascular effects of L. rhinocerotis in rat isolated aortae. Methods Organ bath setup was used to study the effects of L. rhinocerotis cold water extract (CWE) in aortic rings isolated from (8-10) weeks old male Sprague Dawley rats. All the aortic rings were pre-contracted with phenylephrine prior to the construction of concentration-response curve to the extract. The role of endothelium and muscarinic receptor–mediated relaxation of the smooth muscle protocols was performed. Calcium channel inhibitors, 1-[2-(4-Methoxyphenyl)-2-[3-(4-methoxyphenyl)propoxy]ethyl-1H-imidazole hydrochloride (SKF96365) and nifedipine, were used as positive controls to confirm the role of external calcium in eliciting contractions induced by phenylephrine in this preparation. Results Results showed that CWE significantly relaxed the pre-contracted aorta (Emax: 97.70 ± 5.21%). Removal of endothelium or blockade of muscarinic receptor did not affect CWE-induced vasorelaxation. SKF96365 and nifedipine markedly attenuated phenylephrine-induced contractions showing the crucial role of external calcium. Conclusion This is the first study to report the vasorelaxation effects of L. rhinocerotis in rat isolated aorta. These effects are endothelium-independent and likely to be via blockage of calcium channels.

Acknowledgements

This research was supported by Ministry of Higher Education Malaysia Grant (FRGS/1/2014/SKK03/UNIM/02/1). Mei Kee Lee and Kayatri Govindaraju receive scholarships for their PhD studies from the University of Nottingham Malaysia Campus

Keywords: Lignosus rhinocerotis, medicinal mushroom, vascular smooth muscle, Vasorelaxation, organ bath

Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.

Presentation Type: Oral Presentation

Topic: Inflammatory diseases

Citation: Govindaraju K, Lee M, Ting K, Tan C, Ng S, Loh H, Then S and Mohankumar SK (2019). Lignosus rhinocerotis water-soluble sclerotial extract relaxes rat isolated aortae.. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00083

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Received: 18 Oct 2018; Published Online: 17 Jan 2019.

* Correspondence: Prof. Kang Nee Ting, University of Nottingham Malaysia Campus, Department of Biomedical Sciences, University of Nottingham Malaysia Campus, 43500 Semenyih, Malaysia, Semenyih, Malaysia, kang-nee.ting@nottingham.edu.my