Local β-glucan source from oyster mushroom (Pleurotus flabellatus) as potential usage in medical applications
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1
Malaysian Nuclear Agency, Malaysia
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2
Herbal Medicine Research Centre, Institute for Medical Research, Ministry of Health (Malaysia), Malaysia
Background
Many applications of mushroom polysaccharides have been reported such as in drug delivery system, skin applications and as immunomodulatory agents. The polysaccharides can be obtained from the fruit body, mycelium, culture broth and spores of mushroom. In this study, oyster mushroom (Pleurotus flabellatus) culture was produced by submerged culture fermentation due to its higher productivity and faster fermentation rate.
Methods
The biomass obtained from submerged culture fermentation was extracted by using hot water extraction. The polysaccharides were tested using β-glucan kit and also studied using small angle X-ray scattering (SAXS) equipment. Then the polysaccharides were separated using column chromatography before determining the molecular weight using gel permeation chromatography-multi angle laser light scattering (GPC-MALLS).
Results
Both endopolysaccharide and exopolysaccharide obtained have shown the presence of β-glucan (1,3;1,6). The polysaccharides also showed the presence of high molecular weight (~3×10^6 Da) and low molecular weight components. SAXS results showed that the maximum agglomerate size was ~27 nm and the average size of globular was 2.6 nm.
Conclusion
The presence of high molecular weight β-glucan showed potential application as immunomodulatory agents. The polysaccharides can be classified as nanobiomaterials. These studies supported potential usage of local compounds in medical applications.
Keywords:
Pleurotus flabellatus,
Immunomodulation,
Small angle X ray scattering,
β-Glucan,
Submerged fermentation
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Poster Presentation
Topic:
Miscellaneous
Citation:
Mohamad
SA,
Abd Halim
N,
Keong
C,
Mohd Dzomir
A,
Yazid
H,
Hamzah
M and
Nuri
E
(2019). Local β-glucan source from oyster mushroom (Pleurotus flabellatus) as potential usage in medical applications.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00112
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Received:
12 Oct 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Shaiful A Mohamad, Malaysian Nuclear Agency, Kuala Lumpur, Malaysia, shaifulazuar@gmail.com