Pro-inflammatory cytokines expression of carrageenan-induced acute inflammation in rats pre-administered with freeze-dried Carica papaya leaf juice and citropten
Norzahirah
Ahmad1*,
Bee Ping
Teh1,
Nor Azrina
Norahmad1,
Siti Khuzaimah
Maarof1,
Nurul Azreen
Mohd Ismail1,
Umi Rubiah
Sastu@Zakaria1,
Nor Azlina
Zolkifli1,
Azlina
Zulkapli2,
Tiffiny
Ho Chau Dee1,
Nabilah
Md Razak1,
Amirrudin
Muhammad1,
Bazilah
Jusoh1,
Wan Abdul Hakim
Wan Lokman1 and
Mohd Ridzuan
Mohd Abdul Razak1
-
1
Herbal Medicine Research Centre, Institute for Medical Research, Ministry of Health (Malasiya), Malaysia
-
2
Medical Research Resource Centre, Institute for Medical Research, Ministry of Health (Malasiya), Malaysia
Background
Immunomodulatory properties of Carica papaya leaf have been previously highlighted in vitro and in vivo. However, repeated dose pre-treatment effect of C. papaya leaf juice and its compounds in inflammatory model is scarce in the literature. Therefore, this study aims to evaluate the anti-inflammatory effects of freeze-dried C. papaya leaf juice (FD-CPLJ) and citropten pre-administrations in a rat model of carrageenan-induced inflammation.
Methods
Rats in four treatment groups were pre-administered with distilled water (negative control), FD-CPLJ (2,000 mg/kg body weight (BW)), citropten (20 mg/kg BW) and indomethacin as a positive control (10 mg/kg BW) respectively as a single dose for five consecutive days. On Day 5, inflammation was induced by carrageenan injection to the sub-plantar region of the rat’s left hind paw in the treatment groups. Non-carrageenan stimulated groups were pre-administered with distilled water, FD-CPLJ and citropten. Paw volumes were measured using digital water plethysmometer at certain time intervals (one, three, six and 24 hours) post-induction. The paw tissues were harvested and the expression levels of NOS3, CCL2 and TNF genes were quantitated by quantitative reverse transcription PCR. The expression levels of MCP-1 and TNF-α proteins were determined by Western blot analysis.
Results
Paw volume evaluation shows some reduction in the first and third hour post-induction in groups treated with FD-CPLJ and citropten. The TNF-α expression was slightly downregulated in the citropten pre-treated rats but not in the FD-CPLJ pre-treated rats. The expression of NOS3 gene and the expression of MCP-1 protein was downregulated in FD-CPLJ and citropten pre-treated rats. In contrast to MCP-1 result, the expression of CCL-2 was upregulated in the FD-CPLJ pre-treated rats.
Conclusion
Citropten administration shows anti-inflammatory potential which warrants further investigation at different dosages to further elicit its potential. The contrary results in the gene and protein level for FD-CPLJ-treated group requires further investigation into its mechanism of action.
Acknowledgements
This study was supported by the Ministry of Health, Malaysia under project number NMRR-17-507-35387 (JPP-IMR 17-012). We would like to thank the Director General of Health Malaysia for his permission to publish this abstract. The authors also would like to acknowledge those who had contribute directly or indirectly in this study accomplishment. There are no competing financial interests in this study.
Keywords:
cytokine,
Carica papaya,
citropten,
Inflammation,
TNF - α
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Poster Presentation
Topic:
Inflammatory diseases
Citation:
Ahmad
N,
Teh
B,
Norahmad
N,
Maarof
S,
Mohd Ismail
N,
Sastu@Zakaria
U,
Zolkifli
N,
Zulkapli
A,
Ho Chau Dee
T,
Md Razak
N,
Muhammad
A,
Jusoh
B,
Wan Lokman
W and
Mohd Abdul Razak
M
(2019). Pro-inflammatory cytokines expression of carrageenan-induced acute inflammation in rats pre-administered with freeze-dried Carica papaya leaf juice and citropten.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00114
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Received:
16 Oct 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Ms. Norzahirah Ahmad, Herbal Medicine Research Centre, Institute for Medical Research, Ministry of Health (Malasiya), Kuala Lumpur, Malaysia, norzahirah@imr.gov.my