Identification of differentially expressed genes in CaSki cervical cancer cells treated with a selected diarylpentanoid
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1
Jeffrey Cheah School of Medicine and Health Sciences (JCSMHS), Malaysia
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2
Faculty of Science, Putra Malaysia University, Malaysia
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3
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Malaysia
Background
Diarylpentanoids are curcumin analogues with a 5-carbon linker between its aryl rings, showed greater growth inhibitory activity over curcumin. Structural modifications of curcumin, an active component from the root of Curcuma longa has shown to exhibit significant growth-suppressive effect in a wide range of cancer cell lines but its effects on cervical cancer has not been well studied. Our preliminary findings on cytotoxicity and apoptosis indicated that diarylpentanoid (MS17) was able to induce apoptosis and demonstrated antiproliferative effect in human papillomaviruses (HPV) infected cervical cancer cells (CaSki-HPV-16). However, the molecular mechanism underlying this effect is not fully understood and warrants further investigation. The aim of the present study was to identify differentially expressed genes (DEGs) in CaSki following 6µM MS17 treatment for 24 hours.
Methods
Gene expression profile was performed using the Agilent SurePrint G3 Human Gene Expression Array. Functional classification and pathway analysis were performed on the DEGs.
Results
We had identified 104 significantly regulated (p<0.05) DEGs, 45 up- and 59 down-regulated genes in response to MS17 treatment. Functional classification analysis revealed that the up- and down-regulated genes were associated with cell adhesion, cell cycle, chromosome organisation, cytoskeleton organisation, DNA damage response, signal transduction, transcription regulator activity and transporter activity. Our data also revealed that among the DEGs, some up-regulated genes were exclusively associated with oxidation reduction, protein binding, tumour suppressor and unfolded protein response while down-regulated genes were associated with chromosome segregation. Pathways significantly modulated by MS17 were mitotic roles of Polo-Like Kinase, cell cycle: G2/M DNA damage checkpoint regulation, ATM signaling, cyclins and cell cycle regulation, and GADD45 signaling.
Conclusion
The modulation of these genes and associated molecular pathways suggest that MS17 may exhibit its anticancer therapeutic potential activity through cell cycle regulation, growth inhibition and apoptosis on cervical cancer cells.
Keywords:
cervical cancer,
diarylpentanoid,
Gene Expression Profiling,
Curcumin analogue,
Microarray
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Poster Presentation
Topic:
Cancer
Citation:
Naidu
R,
Paulraj
F,
Abas
F,
Lajis
NH and
Othman
I
(2019). Identification of differentially expressed genes in CaSki cervical cancer cells treated with a selected diarylpentanoid.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00121
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Received:
18 Oct 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Rakesh Naidu, Jeffrey Cheah School of Medicine and Health Sciences (JCSMHS), Bandar Sunway, Malaysia, rakesh.naidu@monash.edu