Cell cycle arrest by cowanin on T47D breast cancer cell line
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1
Andalas University, Indonesia
Background
Breast cancer is one of the most common cause of cancer deaths each year. The incidence of cancer is very closely related to cell cycle. Cancer is a disease in which there are irregularities in the regulation of cell cycle. Treatment of cancer depends mainly on chemotherapy. Nevertheless, anticancer agents lack selectivity and are often compromised by onset of resistance.
Methods
The search for new drugs from natural sources is required. This study investigated the effect of cowanin from the stem bark of asam kandis (Garcinia cowa Roxb.) against T47D breast cancer cell cycle by flow cytometry method. Previous study showed that cowanin induced cytotoxic effect at IC50 11.68 µg/mL against T47D breast cancer cells. This study involved three groups, namely the control group, treatment group 1 (cowanin at 1x IC50), and treatment group 2 (2x IC50).
Results
Flow cytometry analysis showed that cowanin inhibited T47D breast cancer at G0-G1 phase. Concentration of 1x IC50 yielded an average percentage of inhibition 54.12% whereas the concentration of 2x IC50 produced an average percentage of inhibition 67.72%. Statistical analysis with one way ANOVA showed that there is significant difference between each treatment group in the percentage of inhibition on G0-G1 phase.
Conclusion
The results indicated that cowanin induced cell cycle arrest in T47D cells, suggesting its potential as a new chemotherapeutic agent
Acknowledgements
The author would like to say special thanks to The Dean of Faculty of Faculty of Pharmacy, Andalas University, Padang, Indonesia for his support this research under Lecturer Research Grant, Fiscal Year 2017.
Keywords:
Cowanin,
Garcinia cowa,
Breast cacner,
Cell cyle,
flow cytoametry,
T47D
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Oral Presentation
Topic:
Cancer
Citation:
Wahyuni
FS,
Arisanty
D and
Yusra
LN
(2019). Cell cycle arrest by cowanin on T47D breast cancer cell line.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00124
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Received:
30 Nov 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Prof. Fatma S Wahyuni, Andalas University, Padang, Indonesia, fatmasriwahyuni@gmail.com