Event Abstract

Antileukaemic effect of palladium nanoparticles mediated by white tea (Camellia sinensis) extract in WEHI-3B cell-induced murine leukaemic model

  • 1 University of Sulaymaniyah, Iraq
  • 2 Putra Malaysia University, Malaysia

Background Cancer nanotherapy is progressing rapidly with the introduction of many innovative nanocarriers using herbal extract as the medium. Palladium nanoparticles (Pd@W.tea NPs), in particular, were reported to exert antiproliferative effects against human leukaemic cells (MOLT-4) through induction of apoptosis and G2/M cell-cycle arrest. As part of the effort in confirming the promising role of Pd@W.tea NPs for treatment against cancers, both in vitro and in vivo antileukaemic effects of Pd@W.tea NPs against WEHI-3B cell-induced murine leukaemia were investigated. Methods Palladium nanoparticles, which was developed by green synthesis using white tea (Camellia sinensis) extract, was characterised by zetasizer, UV–vis spectroscopy, X-ray diffractometry, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The in vitro antileukaemic effect was determined using MTT, Annexin V and cell cycle assays. The in vivo effects of Pd@W.tea NPs in treated leukaemic mice was evaluated through morphological study, histopathological changes and Immunohistochemistry (IHC). Results The results from MTT, Annexin V, and cell cycle assays showed that Pd@W.tea NPs reduced the growth of WEHI-3B leukaemic cells at IC50 of 7.55 µg/mL. Furthermore, outcomes of histopathology and IHC analyses found that the number of leukaemic cells in the spleen of leukaemic mice were significantly lowered to less than 50% after 28 days of oral treatment with of Pd@W.tea NPs at 50 and 100 mg/kg. In addition, western blotting and reverse-transcription quantitative polymerase chain reaction (RT-PCR) assays confirmed the antileukaemic effects of Pd@W.tea NPs through significant (p<0.05) up-regulation of Bax and Cytochrome C genes, with down regulation of Bcl-2 gene. Conclusion Pd@W.tea NPs, which induced an intrinsic apoptotic pathway in murine leukaemia, could be potentially developed into an anticancer agent.

Keywords: Natural Products, Green synthesis, Nanopartical, apoptois, Leukaemia

Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.

Presentation Type: Oral Presentation

Topic: Cancer

Citation: Othman HH (2019). Antileukaemic effect of palladium nanoparticles mediated by white tea (Camellia sinensis) extract in WEHI-3B cell-induced murine leukaemic model. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00126

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Received: 26 Oct 2018; Published Online: 17 Jan 2019.

* Correspondence: Dr. Hemn H Othman, University of Sulaymaniyah, Sulaymaniyah, Iraq, hemnho759604@gmail.com