Antileukaemic effect of palladium nanoparticles mediated by
white tea (Camellia sinensis) extract in WEHI-3B cell-induced murine leukaemic model
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1
University of Sulaymaniyah, Iraq
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2
Putra Malaysia University, Malaysia
Background
Cancer nanotherapy is progressing rapidly with the introduction of many innovative nanocarriers using herbal extract as the medium. Palladium nanoparticles (Pd@W.tea NPs), in particular, were reported to exert antiproliferative effects against human leukaemic cells (MOLT-4) through induction of apoptosis and G2/M cell-cycle arrest. As part of the effort in confirming the promising role of Pd@W.tea NPs for treatment against cancers, both in vitro and in vivo antileukaemic effects of Pd@W.tea NPs against WEHI-3B cell-induced murine leukaemia were investigated.
Methods
Palladium nanoparticles, which was developed by green synthesis using white tea (Camellia sinensis) extract, was characterised by zetasizer, UV–vis spectroscopy, X-ray diffractometry, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The in vitro antileukaemic effect was determined using MTT, Annexin V and cell cycle assays. The in vivo effects of Pd@W.tea NPs in treated leukaemic mice was evaluated through morphological study, histopathological changes and Immunohistochemistry (IHC).
Results
The results from MTT, Annexin V, and cell cycle assays showed that Pd@W.tea NPs reduced the growth of WEHI-3B leukaemic cells at IC50 of 7.55 µg/mL. Furthermore, outcomes of histopathology and IHC analyses found that the number of leukaemic cells in the spleen of leukaemic mice were significantly lowered to less than 50% after 28 days of oral treatment with of Pd@W.tea NPs at 50 and 100 mg/kg. In addition, western blotting and reverse-transcription quantitative polymerase chain reaction (RT-PCR) assays confirmed the antileukaemic effects of Pd@W.tea NPs through significant (p<0.05) up-regulation of Bax and Cytochrome C genes, with down regulation of Bcl-2 gene.
Conclusion
Pd@W.tea NPs, which induced an intrinsic apoptotic pathway in murine leukaemia, could be potentially developed into an anticancer agent.
Keywords:
Natural Products,
Green synthesis,
Nanopartical,
apoptois,
Leukaemia
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Oral Presentation
Topic:
Cancer
Citation:
Othman
HH
(2019). Antileukaemic effect of palladium nanoparticles mediated by
white tea (Camellia sinensis) extract in WEHI-3B cell-induced murine leukaemic model.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00126
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Received:
26 Oct 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Hemn H Othman, University of Sulaymaniyah, Sulaymaniyah, Iraq, hemnho759604@gmail.com