Cytotoxicity of Pogonatum cirratum extracts against CCL-119, a human T-cell acute lymphoblastic leukaemia cell line
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1
National University of Malaysia, Malaysia
Background
Acute lymphoblastic leukaemia (ALL), the most common form of childhood cancer, is characterised by abnormal differentiation, proliferation, and accumulation of lymphoid progenitor cells in the bone marrow and extramedullary sites. Given the various harmful side effects associated with current treatment regimens of ALL, natural products have become the focus of on-going search for alternative anti-acute leukaemic agents. Bryophyte mosses that possess medicinal values have yet been fully explored. Thus, this study aimed to investigate phytochemicals and cytotoxic activity of Pogonatum cirratum subsp. macrophyllum extracts against CCL-119, a human T-cell acute lymphoblastic leukaemia cell line.
Methods
P. cirratum was extracted using three solvents with different polarity; aqueous, methanol and petroleum ether. Each extract was then subjected to chemical screening of phytochemical contents. CCL-119 cells were treated with each extract at different concentrations ranging from 0-200µg/mL for 48 hours. Dexamethasone (Dex) was used as positive control. Cytotoxic effects and apoptotic activities of the extracts against CCL-119 ALL cell line were assessed using colorimetric MTT assays and Annexin V/ Propidium iodide staining, respectively.
Results
The percentage yield of aqueous, methanol and petroleum ether extracts were 1.4%, 6.25% and 0.38%, respectively. The extracts were tested positive for flavonoid, glycoside, phenol and saponin. P. cirratum exerted cytotoxic effects and apoptotic activities against CCL-119 cells. Aqueous extract of P. cirratum was the most potent (IC50= 4.57±0.33µg/mL) followed by methanol (IC50= 6.0±0.29µg/mL) and petroleum ether (IC50= 20.5±1.51µg/mL) extracts. Primary cell death was mediated via apoptosis with percentage of 90.5±2.85%, 82.7±3.03% and 69.7±1.23% in aqueous, methanol and petroleum ether extracts-treated cells, respectively. Dex-treated cells showed IC50 at 0.08x10-2±2.85µM, and cell death was mediated via apoptosis (44.1±2.11%) and necrosis (9.0±0.67%).
Conclusion
P. cirratum-induced cytotoxicity by apoptosis in cultured acute leukaemic cells could serve as the scientific basis for its usefulness as potential safer chemotherapeutic agent, particularly for childhood ALL.
Acknowledgements
UKM Moss Herbarium researchers and technical staff at Biology Building, Faculty of Science an Technology
Keywords:
bryophyte pogonatum,
medicinal value moss,
childhood T-cell ALL,
Cytotoxicity,
Apoptosis
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Poster Presentation
Topic:
Cancer
Citation:
Latif
ES,
Kamaludin
NF and
Rajasegaran
R
(2019). Cytotoxicity of Pogonatum cirratum extracts against CCL-119, a human T-cell acute lymphoblastic leukaemia cell line.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00136
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Received:
30 Sep 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Elda S Latif, National University of Malaysia, Bangi, Malaysia, elda.latif@ukm.edu.my