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Biological evaluation of nano-colloidal carrier loaded with cyclosporine, potentially for topical treatment of psoriasis

Norazlinaliza Salim1*, Siti Hajar Musa1 and Norashikin Shamsudin2
  • 1 Integrated Chemical BioPhysics Research, Faculty of Science, Universiti Putra Malaysia, Malaysia
  • 2 Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia

Background Psoriasis is known as a type of skin disease which easily spotted as dry skin with red and thick patches over the body. This has become a major problem for the patients as this skin type give uncomfortable feeling and a very itchy skin condition. This skin problem is a lifetime disease with no infinite cure due to the ineffectiveness of the drug release. In this study, the newly nanoemulsions formulation containing cyclosporine (an antipsoriatic drug) could be potential for psoriasis patients. Methods Cyclosporine-loaded nanoemulsion has been developed by a combination of low and high energy emulsification techniques. The formulation was then further evaluated on toxicity, colony counting, transepidermal water loss (TEWL), skin moisturizing effect and irritancy test. Results From the results obtained, when cyclosporine was incorporated with the nanoemulsion carrier, its toxicity has been reduced. Their toxicity was recorded to be above the IC50 limit. In colony count analysis, results showed that after the spreading of nanoemulsion on the nutrient agar plate, it was found to have none of colony growth after 16-18 h of analysis. In TEWL and level of water content analysis showed that all volunteers gave significant positive results (P<0.05) with the reduction of TEWL as well as in skin’s water storage by all 15 volunteers. The highest increment of water uptake was 68.87 %. In irritancy analysis, the HIE score did not showed any irritant possibility towards the protein reagent. In addition, survey has also been carried out towards all volunteers on the effect of after 3 h application. None of them have reported to experience any skin irritancy, skin burning, skin inflammation or skin scaling. Conclusion Cyclosporine-loaded nanoemulsions have a good potential to be used for topical treatment of Psoriasis with good water storage for all volunteers’ skin with no record of irritancy side effect.

Acknowledgements

The financial assistance provided by Universiti Putra Malaysia under Putra Grant (GP-IPS/2016/9481700) was gratefully acknowledged.

References

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Keywords: Psoriasis, Cyclosporine, topical treatment, Nanoemulsion, Colloidal carrier

Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.

Presentation Type: Oral Presentation

Topic: Miscellaneous

Citation: Salim N, Musa S and Shamsudin N (2019). Biological evaluation of nano-colloidal carrier loaded with cyclosporine, potentially for topical treatment of psoriasis. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00147

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Received: 22 Nov 2018; Published Online: 17 Jan 2019.

* Correspondence: Dr. Norazlinaliza Salim, Integrated Chemical BioPhysics Research, Faculty of Science, Universiti Putra Malaysia, Seri Kembangan, Malaysia, azlinalizas@upm.edu.my