Drug-herb interaction between methanolic and aqueous extracts of Clinacanthus nutans and cisplatin on breast cancer cell line MCF-7
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1
Universiti Teknologi MARA Puncak Alam, Malaysia
Background
Drug-herb interactions may affect drug therapy in several ways leading to beneficial or even harmful consequences. Significant numbers of patients all around the world consume natural products as complementary to conventional medicine in the battle against various diseases including cancer. Clinacanthus nutans locally known as Sabah snake grass is popular for its anti-cancer properties and taken as prevention as well as treatment for cancer. It is crucial to evaluate potential antagonistic, additive or synergistic interactions that may result from co-treatment of this plant in chemotherapy.
Methods
In this study, after preliminary cytotoxicity test of methanolic (MCN) and aqueous (WCN) extracts of C. nutans on a panel of cell lines was conducted. MTT assay was used to test single treatment as well as the combination of extracts and cisplatin (CP) on MCF-7, breast cancer cell line. From the data, combination indexes were calculated and isobolograms were constructed.
Results
CP, MCN and WCN decreased the survival of breast cancer cells with IC50 of 43.59 µM, 378.44 µg/mL and 910.92 µg/mL respectively. Combination of WCN and CP exhibited strong antagonism. Most combination of MCN and CP also generated significant antagonistic effect except for combination of CP with high concentration of MCN which is additive (CI=1).
Conclusion
MCN has greater cytotoxic affect against MCF-7 compared to WCN and both showed strong antagonism when used in combination with CP.
Keywords:
Clinacanthus nutans,
Combination index,
Isobologram,
MCF-7,
Cancer
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Poster Presentation
Topic:
Cancer
Citation:
Abd Mutalib
N,
Abd Latip
N,
Mohd Nawawi
F and
Osman
N
(2019). Drug-herb interaction between methanolic and aqueous extracts of Clinacanthus nutans and cisplatin on breast cancer cell line MCF-7.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00150
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Received:
16 Oct 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Normala Abd Latip, Universiti Teknologi MARA Puncak Alam, Selangor, Selangor, 42300, Malaysia, drnormala6351@puncakalam.uitm.edu.my