Quantitative analysis of andrographolide, a biomarker of Andrographis paniculata, using qNMR spectroscopy
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1
School of Pharmacy, International Medical University, Bukit Jalil, 57000, Department of Pharmaceutical Chemistry, Malaysia
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2
School of Pharmacy, International Medical University, Bukit Jalil, 57000, Malaysia
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3
Institute of Science, Universiti Teknologi MARA (UiTM), 40450, Organic Synthesis Laboratory, Malaysia
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4
Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Pharmacotherapeutics Unit, Department of Medicine, Malaysia
Background
Andrographis paniculata also known as “king of bitters”, is a well-known traditional medicinal herb which has been widely used in Asian countries. Andrographolide (AGP) is the major bioactive constituent of A. paniculata and a potent biomarker which has been shown to have a wide range of pharmacological properties including anti-inflammatory, antitumor and hypoglycemic activities. In this study, 1H NMR spectroscopy has been successfully introduced to quantify the amount of AGP present in herbal preparations.
Methods
1H NMR based quantification approach was established via optimisation of experimental conditions, including NMR pulse sequence and NMR relaxation delay time. The NMR spectra were obtained in dimethylsulfoxide-d6 using maleic acid as the internal standard.
Results
The results indicate that qNMR spectroscopy is a powerful analytical tool for rapid and accurate determination of AGP in herbal preparations. The developed method was found to be linear (r2 = 0.9983) and the percentage recovery for the accuracy was 98.88% - 99.38%. The %RSD for precision, robustness and stability studies was found to be less than 2%. The method has also been successfully applied to test few commercial preparations of A. paniculata sold in the local market.
Conclusion
The qNMR spectroscopy is found to be a rapid and versatile tool for the qualitative and quantitative determination of AGP as well as impurities in herbal preparations.
Keywords:
Andrograhis paniculata,
andrographolide,
quantification,
NMR,
Herbal
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Oral Presentation
Topic:
Miscellaneous
Citation:
Sagineedu
S,
Kong
K,
Phang
S,
Toh
B,
Chu
V,
Hamzah
A and
Stanslas
J
(2019). Quantitative analysis of andrographolide, a biomarker of Andrographis paniculata, using qNMR spectroscopy.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2019.63.00009
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Received:
29 Nov 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Sreenivasa Rao Sagineedu, School of Pharmacy, International Medical University, Bukit Jalil, 57000, Department of Pharmaceutical Chemistry, Kuala Lumpur, Malaysia, sreenivasarao@imu.edu.my