The effect of administration of an equal dose of different classes of dietary chemicals on heme oxygenase-1 gene expression in mice liver
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1
Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Malaysia
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2
University of Tripoli, Libya
Background
Heme oxygenase-1 (HO-1) is an enzyme that possesses anti-oxidant, anti-inflammatory and cytoprotective functions. lnduction of HO-1 occurs as an adaptive and beneficial response to various injurious stimuli such as oxidative stress. This study is aimed at monitoring the effects of equal doses (50 mg/kg) of sulforaphane, curcumin, quercetin, indole-3-carbinol and butylated hydroxyanisole on the expression levels of HO-1 gene.
Methods
48 adult male ICR white mice (25–30 g) were divided into 8 groups: normal control group (n=6), sulforaphane treated group (n=6), quercetin treated group (n=6), curcumin treated group (n=6), butylated hydroxyanisole treated group (n=6), indole-3-carbinol treated group (n=6), vehicle 1 control group (n=6) and vehicle 2 control group (n=6). All chemicals were administered intraperitoneally at a dose of 50 mg/kg for 14 days. Vehicle 1 (DMSO, Tween 20 and normal saline at a ratio of 0.05:0.1:0.85) was used to dissolve sulforaphane, quercetin and curcumin. Vehicle 2 (corn oil) was used to dissolve indole-3-carbinol and butylated hydroxyanisole. At day 15, the animals were sacrificed and their livers were isolated. From the liver, total RNA was extracted, reverse transcribed and subjected to quantitative real‐time PCR to detect HO-1 gene expression. Agarose gel electrophoresis was also performed to verify the specificity of the amplification.
Results
Our results showed that there were 4.6, 3.6, 3.6, 3.3 and 3-fold significant increases in HO-1 gene expression levels after treatment with 50 mg/kg of sulforaphane, indole-3-carbinol, butylated hydroxyanisole, curcumin and quercetin respectively, compared to controls (p<0.05).
Conclusion
At a dose of 50mg/kg, sulforaphane administration for 14 days resulted in the highest induction of HO-1 gene expression level in mice liver, and quercetin the lowest.
Keywords:
Heme Oxygenase-1,
Sulforaphane,
Quercetin,
Curcumin,
Indole-3-carbinol,
Butylated Hydroxyanisole,
Gene Expression
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Poster Presentation
Topic:
Miscellaneous
Citation:
Abdullah
A,
Alrawaiq
NS and
Elbadri
A
(2019). The effect of administration of an equal dose of different classes of dietary chemicals on heme oxygenase-1 gene expression in mice liver.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2019.63.00029
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Received:
27 Sep 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Azman Abdullah, Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Malaysia, manlah1969@yahoo.com