Interactive mouse brain atlas of tetracycline-responsive calmodulin-dependent protein kinase II- and prion protein promoter expression
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1
Nordlandssykehuset Bodo, Department of Neurology, Norway
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2
University of Tubingen, Department of Medical Genetics, Germany
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3
University of Oslo, CMBN & IMB, Norway
Conditional transgenic mouse models are increasingly employed for investigations of neurodegenerative diseases, and evaluation of potential therapeutic interventions. The most commonly used conditional transgenic system is the tetracycline responsive gene (Tet-Off) system. In this system, the expression of the gene of interest is dependent on a Tetracyclin-regulatable transactivator which is expressed under the control of a specific promoter construct. Prior knowledge about the spatial distribution of the regulated gene expression in the brain is critical for the successful construction of conditional disease models as well as subsequent interpretation of findings. Gene expression distributions can be investigated in detail using double transgenic mice in which the promoter construct regulates a LacZ reporter gene encoding the histologically detectible marker beta-galactosidase. However, investigations of distribution patterns in histological materials are challenging since 1) expert knowledge is needed to determine anatomical locations, 2) communication of findings in conventional journal publications is limited by format and space restrictions, and 3) comparisons of distributions between different mouse lines require extensive anatomical mapping to identify corresponding locations. Building upon earlier digital brain atlas systems developed within the Rodent Brain Workbench portal (www.rbwb.org) we have developed a novel atlas application system tailored for side-by-side comparison of spatially corresponding histological section images. The atlas contains comprehensive series of anatomically annotated high-resolution section images obtained from the two most commonly used Tet-Off promoter mouse lines, the calmodulin-dependent protein kinase II (CamKII) promoter line, and the prion protein (PrP) promoter line. An interactive dual image viewer tool allows panning and zooming of spatially corresponding image pairs from the two animal models, and a comprehensive table of brain regions containing labeling provides efficient access to corresponding atlas images through a selected region of interest. Analyses of spatial distributions show considerable differences in the spatial expression patterns between the two lines both at the regional and cellular level, relevant for investigators working with the CamKII and PrP Tet-Off promoter mouse lines.
Conference:
Neuroinformatics 2009, Pilsen, Czechia, 6 Sep - 8 Sep, 2009.
Presentation Type:
Poster Presentation
Topic:
Digital atlasing
Citation:
Odeh
F,
Schmidt
T,
Boy
J,
Moene
IA,
Riess
O,
Bjaalie
JG and
Leergaard
TB
(2019). Interactive mouse brain atlas of tetracycline-responsive calmodulin-dependent protein kinase II- and prion protein promoter expression.
Front. Neuroinform.
Conference Abstract:
Neuroinformatics 2009.
doi: 10.3389/conf.neuro.11.2009.08.012
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Received:
21 May 2009;
Published Online:
09 May 2019.
*
Correspondence:
Francis Odeh, Nordlandssykehuset Bodo, Department of Neurology, Bodo, Norway, u.r.f.odeh@medisin.uio.no