Measuring the effect of a Tris-Indolyl Compound on PC3 Cells
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1
Georgia Southern University, United States
The ability of Compound B to reduce the viability of prostate cancer cells was tested via MTS cell viability assays on PC3 (Prostate Cancer cell line).
Compound B, a novel tris-indolyl iron chelating compound, was tested by the National Cancer Institute’s Developmental Therapeutics Program and shown to have anticancer activity. Building upon these finding, Compound B was synthesis in our lab and further testing was done. Here, MTS assays were used to measure Compound B’s effect on the viability PC3 cells at varying concentration. Results showed that PC3 cells exposed to Compound B at a 69.8 μM concentration showed a 40% decrease in viability.
Acknowledgements
Cell culture facilities were provided by the GSU Department of Chemistry & Biochemistry. Flow Cytometry facilities were provided by GSU Department of Biology
References
Sears,R.B.,Carpenter,R.A., Whitlock,C.R, A General Synthesis of Tris-Indole Derivatives as Potential Iron Chelators, Molecules 2005, 10, 488-491
Keywords:
PC3 cancer cell line,
Anticancer properties,
Iron chelators,
MTS assay,
Apoptosis assay,
tris-indolyl
Conference:
National Organization for the Professional Advancement of Black Chemists and Chemical Engineers (NOBCChE) 45th Annual Conference , Orlando, Florida, United States, 17 Sep - 20 Sep, 2018.
Presentation Type:
Poster Presentation
Topic:
Medicinal Chemistry
Citation:
Wallace
AF,
Tolentino
T and
Whitlock
C
(2019). Measuring the effect of a Tris-Indolyl Compound on PC3 Cells.
Front. Chem.
Conference Abstract:
National Organization for the Professional Advancement of Black Chemists and Chemical Engineers (NOBCChE) 45th Annual Conference .
doi: 10.3389/conf.fchem.2018.01.00061
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Received:
18 Oct 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Timothy Tolentino, Georgia Southern University, Statesboro, United States, ttolentino@georgiasouthern.edu
Dr. Christine Whitlock, Georgia Southern University, Statesboro, United States, cwhitlock@georgiasouthern.edu