Real-time observation of biomineralization process of calcium phosphate nanocrystals
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1
University of Illinois at Chicago, Department of Mechanical Engineering, United States
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2
Shandong University, China
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3
University of Illinois at Chicago, College of Dentistry, United States
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4
University of Illinois at Chicago, Department of BioEngineering, United States
Calcium phosphate, which is the major component of bone and teeth, is a biomineral closely related to our daily life. The thorough understanding of the calcium phosphate biomineralization process is critical to the orthopedics and dentistry surgery. The biomineralization process can be chronologically divided to five stages featuring ions, prenuclei cluster, nuclei, amorphous phase and crystal phase stages, respectively. Many techniques have been used to study the reactions within biosimulated aqueous solution, most of these techniques, however, cannot clearly demonstrate the underlying mechanisms. The detailed biomineralization reactions, especially that happen during the earlier stages, are hard to be observed by any conventional microscopy methods. One reliable technique should have both high time and spatial resolutions, ransmission electron microscopy (TEM) equipped with in-situ liquid holder, can provide real time observation up to nanoscale resolution resolved at 30 fps (frames per second). In this research, using in-situ liquid TEM, we explored the biomineralization of calcium phosphate. The dynamics and growth of calcium phosphate particles at the early stages can be recorded. We observed different stages of biomineralization (ions – prenuclei cluster – nuclei – amorphous phase). We also monitored the transformation of amorphous calcium phosphate to crystalline HA after extended period of time.
Reza Shahbazian-Yassar thanks financial support from NSF Award No 1100806).
Keywords:
biomaterial,
Calcium phosphate,
Imaging method,
Nano/micro particle
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Surface and interfacial characterization
Citation:
Shahbazian-Yassar
R,
He
K,
Shokuhfar
T,
Lu
Y,
Sukotjo
C,
Nie
A and
Yuan
Y
(2016). Real-time observation of biomineralization process of calcium phosphate nanocrystals.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.00126
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.