3D tissue printing
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1
Harvard University, School of Engineering and Applied Sciences, United States
The advancement of tissue and, ultimately, organ engineering requires the ability to pattern human tissues composed of cells, extracellular matrix, and vasculature with controlled microenvironments that can be sustained over prolonged time periods. To date, bioprinting methods have yielded thin tissues that only survive for short durations. To improve their physiological relevance, we report a method for bioprinting 3D cell-laden, vascularized tissues that exceed 1 cm in thickness and can be perfused on chip for long time periods (> 6 weeks). Specifically, we integrate parenchyma, stroma, and endothelium into a single thick tissue by co-printing multiple inks composed of human mesenchymal stem cells (hMSCs) and human neonatal dermal fibroblasts (hNDFs) within a customized extracellular matrix alongside embedded vasculature, which is subsequently lined with human umbilical vein endothelial cells (HUVECs). These thick vascularized tissues are actively perfused with growth factors to differentiate hMSCs toward an osteogenic lineage in situ. This longitudinal study of emergent biological phenomena in complex microenvironments represents a foundational step in human tissue generation. We then describe how this approach can be extended to create other functional tissue constructs, such as 3D proximal tubules, on chip for drug screening, disease models, and ultimately tissue repair and regeneration.
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
New Frontier Oral
Topic:
Biomaterials in constructing tissue substitutes
Citation:
Lewis
JA
(2016). 3D tissue printing.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.00257
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Received:
28 Mar 2016;
Published Online:
30 Mar 2016.