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Cytotoxicity analysis of hydrogels based on chitosan incorporated with variable amounts of silver nanoparticles

Katarzyna Bialik-Wąs1, Bozena Tyliszczak1, Dorota Walczyk1, Dagmara Malina2 and Agnieszka Sobczak-Kupiec2
  • 1 Department of Chemistry and Technology of Polymers, Cracow University of Technology, Poland
  • 2 Institute of Inorganic Chemistry and Technology, Cracow University of Technology, Poland

Recently, greater attention is devoted towards development  of modern polymeric biomaterials of  particular applications, mainly hydrogels defined also as superabsorbent polymers (SAP). Hydrogels based on chitosan and natural polymers constitute significant source of biocompatible, biodegradable matrices widely applied in biomedicine. The main assets of biomaterials constitute: biocompatibility, lack of allergic, toxic, mutagenic reactions, lack of immune response and inflammations[1]-[4].

In this study chitosan based hydrogels were formed by exposure to UV light, applying PEGDA 700 as crosslinking agent  and 2-hydroxy-2-methylpropiophenone as photoinitiator. Initial mixture exposed to UV-light was comprised of 3% of chitosan, 2% of gelatin and accordingly: 1ml (1), 3ml (2) and 5ml (3)  of silver nanoparticles (250ppm). Another samples 4 and 5 contained the same reagents as sample 2 and 3 but were enriched in 5ml of 0.3% aqueous solution of albumin.

The main objective of performed analyses was based on evaluation of cytotoxicity performed on the cells of human dermis in vitro (BJ, ATCCÒ CRL-2522TM)  according to MTT and  XTT methods. The attained results are listed in tables 1 and 2.

Table 1. Determination of cytotoxicity of hydrogel's extracts on human dermis cells applying MTT method.

Table 2. Determination of cytotoxicity of hydrogel's extracts on human dermis cells applying XTT method.

Received hydrogel matrices were subjected to analyses of swelling ability and incubation studies in distilled water and simulated body fluids. The morphology and dispersion of silver nanoparticles in three dimensional structure of hydrogels was determined by means of SEM equipped with EDS analysis. Moreover, the structures of received hydrogels were evaluated applying FT-IR spectroscopy. The positive values of the cytotoxicity analysis indicate formation of innovative hydrogel matrices with enhanced antibacterial activity.

“The authors would like to thank the The National Centre for Research and Development (Grant no: LIDER//033/697/L-5/13/NCBR/2014) for providing financial support to this project”.

References:
[1] N.A. Peppas, P. Bures, W. Leobandung, H. Ichikawa, European Journal of Pharmaceutics and Biopharmaceutics, 2000, 50, 27-46.
[2] J. Zhu, Biomaterials, 2010, 31, 4639-4656.
[3] Riccardo A.A. Muzzarelli, Carbohydrate Polymers 76 (2009) 167–182.
[4] Marguerite Rinaudo, Prog. Polym. Sci. 31 (2006) 603–632.

Keywords: Hydrogel, in vitro, Bioactivity, Polymeric material

Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.

Presentation Type: Poster

Topic: Regeneration inducing biomaterials

Citation: Bialik-Wąs K, Tyliszczak B, Walczyk D, Malina D and Sobczak-Kupiec A (2016). Cytotoxicity analysis of hydrogels based on chitosan incorporated with variable amounts of silver nanoparticles. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00456

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Received: 27 Mar 2016; Published Online: 30 Mar 2016.