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Effect of 3% 4-hexylresorcinol silk vascular patch on healing of injured carotid artery in a rat model

Min Keun Kim1, Chang Hwan Kim1, Young Wook Park1, Hae-Yong Kweun2 and Seong Gon Kim1
  • 1 College of dentistry, Gangneung Wonju National University, Department of oral and maxillofacial surgery, Korea
  • 2 National Academy of Agricultural Science, Sericultural & Apicultural Materials Division, Korea

Aims and Objectives: The aim of this study is to determine the effect of 3% 4-hexylresorcinol(4HR) silk vascular patch as a vascular repairing material on the injured carotid artery of the rat. The other objective is to analysis of the functional results of the healed carotid arteries between direct closure group and vascular patch groups.

Materials and Methods: We used the 21 Sprague-Dawley rats with 8 weeks old and body weights of 250~300 g. The right caoritd artery of every rats were excised with sharp scissor, the defect size was 1 x 0.5 mm. And then we applicate 4-hr silk membrane(Group A) and GORE® ACUSEAL Cardiovascular Patch(Group B), and direct closrue(Group C). We use ultrasonic device for compare the peak systolic velosity, (PSV), end diastolic velosity(EDV) and rdsistivity index(RI) of all groups. And we use the angiography of carotid artery to confirm the patency of the injured carotid artery. After the carotid angiography, we get the tissue sample and histologic analysis was done.

Results: The 4-HR silk membrane show good success rate of re-anastomosis than direct closure and gore-tex vascualr patch group. At the histologic analysis, 4-HR group show better result than gore tex group with minimal foreign body reactions. In gore tex group, there was 2 foreign body granulomas. At the ultrasonographic analysis, direct closure group show higher velosities than vascular patch groups.

Discussion and Conclusion: In this experiment, the potential of 4-hr silk membrane as a possible candidate for vascular patches to accelerate vascular wall regeneration was discovered. The silk membrane can help regeneration of injured carotid artery and show good result compared with gore-tex vascular parch.

Key words: Rat, carotid artery, microsurgery, vascular patch, gore-tex, 4-HR silk membrane, direct closure.

Acknowledgements: This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ011214042016) of the Rural Development Administration, Republic of Korea.

This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ011214042016) of the Rural Development Administration, Republic of Korea.

References:
[1] Heller KS, Strong EW. Carotid arterial hemorrhage after radical head and neck surgery. The American Journal of Surgery 1979;138:607-10.

Keywords: Cell Adhesion, Regenerative Medicine, biomaterial, tissue compatibility

Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.

Presentation Type: Poster

Topic: Biomaterials in thrombosis and hemostasis

Citation: Kim M, Kim C, Park Y, Kweun H and Kim S (2016). Effect of 3% 4-hexylresorcinol silk vascular patch on healing of injured carotid artery in a rat model. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00597

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Received: 27 Mar 2016; Published Online: 30 Mar 2016.