Highly performance OECTs made by inkjet-printing for glucose sensing on paper
-
1
Ecole Nationale supérieure des Mines de saint Etienne, department of bioelectronics, France
-
2
Ecole Nationale supérieure des Mines de saint Etienne, department of flexible electronics, France
-
3
Microvitae technologies, France
-
4
University of Nebraska – Lincoln, Biological Systems Engineering, United States
Organic electrochemical transistors (OECTs) have recently gained a great interest due to their advantages such as ease of fabrication, biocompatibility. PEDOT.PSS is a conducting polymer commonly used for its mixed ionic and electronic transport properties that make it an ideal material for interfacing electronics with biology. PEDOT.PSS is usually spin-coated and then patterned using subtractive techniques such as lift-off or laser ablation. Recently, PEDOT.PSS has been printed using an additive technology, by inkjet. Inkjet is a low cost technology that is compatible with a wide range of substrates such as paper, an ecofriendly and inexpensive alternative for plastics to make disposable biosensors. In this work, we focused on inkjet printing the device to detect the presence of glucose using an electron mediator, Ferrocene, in presence of the associated enzyme the glucose oxidase (GOx) (fig.1).
To fit with inkjet rheology requirements, a new PEDOT.PSS ink has been formulated and successfully printed with a Dimatix Materials Printer. Thin-films can be obtained after curing at 160°C for 30 min. We functionalized the gate, printing on top of it an ink comprising chitosan/ferrocene, an electron mediator. We added glucose oxidase (GOx) in the electrolyte solution of phosphate buffered saline. The channel and gate dimensions (length and width) were respectively 5mmx1mm and 5mmx2mm (fig. 2)
.
We prepared the electron mediator ink by mixing 15mg chitosan/ferrocene in 5mL acetic acid (0.3%wt) with a surfactant and a flush solution (water and ethylene glycol). We placed a polydimethylsiloxane (PDMS) well on top of the transistor and added the electrolyte mixed with the enzyme. After stabilization of the drain current, we added successively different concentrations of glucose solutions resulting in different device responses (fig. 3).
We fabricate inkjet printed OECTs with an electrical conductivity up to 420 S/cm. In our work, we detect the presence of glucose inducing the enzyme specific reaction of glucose coupled with ferrocene an electron mediator[1]. Ferrocene acts as an electron shuttle from the GOx to the PEDOT:PSS gate, causing de-doping of the channel and then the drain current. This decrease varied accordingly with the glucose concentration in the solution. Results exhibit a detection range between 2.70 and 10.00 mM after additions of different concentrations of glucose which is consistent with that concentration in blood[2].
In this study, PEDOT:PSS and chitosan/ferrocene –based inks were successfully printed on paper towards the development of an all printed paper OECT based glucose sensor. The detection of glucose was successful in a range of concentrations, similar to that in human blood. These results demonstrate that inkjet-printing can be used as a facile and low-cost technique to fabricate high quality OECTs. Those results pave the way to establish a paper-based, simple and low cost biosensing platform suitable for point of care diagnostics.
Mary Donahue; Xenofon Strakosas
References:
[1] Shim N. et al. Sensors. 2009, 9, 9896-9902
[2] Panchbhai A.S. et al. J. Oral. Maxillofac. Res. 2012, 3(3):e3,
Keywords:
biosensing,
polymer,
Bioprinting
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Biomaterials in printing
Citation:
Bihar
E,
Pappa
A,
Davidson
C,
Curto
V,
Saadaoui
M and
Malliaras
G
(2016). Highly performance OECTs made by inkjet-printing for glucose sensing on paper.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.00602
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
27 Mar 2016;
Published Online:
30 Mar 2016.