Expansion of adipose-derived stem cells with FGF2 up-regulates mesenchymal markers and modifies osteogenic differentiation potential
-
1
Medical University of Warsaw, Department of Histology and Embryology, Centre for Biostructure Research, Poland
-
2
Centre for Preclinical Research and Technology, Poland
-
3
Medical University of Warsaw, Postgraduate School of Molecular Medicine, Poland
FGF-2 is a potent mitogen but it’s effect on osteogenesis of mesenchymal stem cells is ambiguous. We studied the influence of FGF-2 added at the expansion stage on adipose derived stem cells (ADSC). Human ADSC were cultured in medium with or without FGF-2. Focal adhesion, immunophenotype and mesenchymal transcription factors were analyzed. Cells were further exposed to osteogenic medium (OM) without FGF-2 - ALP activity, mineralization and osteogenic genes expression were investigated. ADSC mesenchymal immunophenotype was confirmed regardless of FGF-2 - at least 95% of cells were CD166+, CD105+, CD90+ and CD73+ and CD45-, CD31- (flow cytometry). However, FGF-2 up‑regulated Sox2 and W5C5 - both stem cell characteristic proteins. Furthermore, FGF-2 increased vinculin organization into focal adhesion points and up-regulated gene expression of mesenchymal stem cell transcription factors HoxA5 and HoxB6, as well as FZD1 - Wnt ligand receptor. Interestingly, expression of both osteogenesis and adipogenesis master regulators, Runx2 and PPARγ, was increased due to FGF-2. FGF-2-precultured cells displayed also increased ALP activity and enhanced mineralization on day 7 and 14 in OM. On day 7, Runx2, ALP and PDPN genes expression was up-regulated, while osteopontin was down-regulated and collagen I was not affected. Increased expression of stem cell characteristic markers, osteogenesis and adipogenesis master regulators and Wnt signaling receptor suggests, that pretreatment in FGF-2 increases the stemness of ADSC. It also promotes osteogenic differentiation, however down-regulation of osteopontin gene expression in the FGF‑2‑‑pretreated population, suggests that FGF-2 might alter the molecular mechanisms of ADSC osteogenic differentiation.
Keywords:
Cell Differentiation,
Cell Proliferation,
cell fate,
cell phenotype
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Biomaterials in mesenchymal and hematopoietic stem cell biology
Citation:
Ruminski
S,
Pawlos
D and
Lewandowska-Szumiel
M
(2016). Expansion of adipose-derived stem cells with FGF2 up-regulates mesenchymal markers and modifies osteogenic differentiation potential.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.00634
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
27 Mar 2016;
Published Online:
30 Mar 2016.