Modulation of keratin in adhesion, proliferation, adipogenic and osteogenic differentiation of porcine adipose-derived stem cells
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1
National Taiwan University, Chemical Engineering, Taiwan
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2
Taipei Medical University, Taiwan
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3
National Taiwan University Hospital, Surgery, Taiwan
Keratin, isolated from human hair, gains researcher’s interests due its intrinsic ability to interact with different cells. Keratin has the potential to serve as a controllable extracellular matrix protein and can be used to demonstrate cell mechanism and cell-matrix interaction. However, the effects of keratin on stem cells have not been reported. In the present study, the effects of keratin on porcine adipose-derived stem cells (pASCs) and a series of selective cell lines: 3T3 fibroblasts, Madin-Darby canine kidney (MDCK) cells, and MG63 osteoblasts were demonstrated. Relative to un-treated culture plate, results showed that keratin coating substrates promoted cell adhesion and proliferation to above cell lines. Keratin also improved pASCs adhesion, proliferation, and enhanced cell viability. Evaluation of genetic markers showed that adipogenic and osteogenic differentiations of pASCs can be successfully induced, which revealed the keratin did not influence the stemness of pASCs. In addition, keratin improved adipogenic differentiations in terms of up-regulations in lipoprotein lipase, peroxisome proliferator-activated receptor gamma and CCAAT-enhancer-binding protein alpha. The osteogenic markers type I collagen, runt-related transcription factor 2, and vitamin D receptor were also upregulated on keratin substrates. Therefore, keratin can serve as a biological derived material for surface modification and scaffold fabrication for biomedical purpose. The combination of keratin with stem cells may be a potential candidate for tissue repair in the field of regenerative medicine.
MOST 102-2221-E-002-039
References:
[1] Verma V, Verma P, Ray P, Ray AR. Preparation of scaffolds from human hair proteins for tissue-engineering applications. Biomed Mater 2008;3(2):025007.
[2] Magin TM, Vijayaraj P, Leube RE. Structural and regulatory functions of keratins. Exp Cell Res 2007;313(10):2021-32.
[3] Richter JR, de Guzman RC, Van Dyke ME. Mechanisms of hepatocyte attachment to keratin biomaterials. Biomaterials 2011;32(30):7555-61.
Keywords:
Cell Differentiation,
stem cell,
Scaffold,
biomacromolecule
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Biomaterials and cellular signaling
Citation:
Yu
J,
Yang
K,
Wu
Y,
Lin
C and
Cheng
N
(2016). Modulation of keratin in adhesion, proliferation, adipogenic and osteogenic differentiation of porcine adipose-derived stem cells.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.01203
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.
*
Correspondence:
Dr. Jiashing Yu, National Taiwan University, Chemical Engineering, Taipei, Taiwan, Email1
Dr. Yen-Lin Wu, National Taiwan University, Chemical Engineering, Taipei, Taiwan, nui718@hotmail.com
Dr. Che-Wei Lin, National Taiwan University, Chemical Engineering, Taipei, Taiwan, d01642002@ntu.edu.tw
Dr. Nai-Chen Cheng, National Taiwan University Hospital, Surgery, Taipei, Taiwan, naichenc@gmail.com