Multi-channeled gelatin scaffold incorporated with neurotrophic gradient and nanotopography as nerve guidance conduit for peripheral nerve regeneration
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1
National Tsing Hua University, Department of Materials Science and Engineering, Taiwan
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2
Tatung University, Department of Material Engineering, Taiwan
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3
Tzu Chi General Hospital, Department of Neurosurgery, Taiwan
Artificial nerve conduits can be used as nerve grafts to enhance the regeneration of large nerve defects. Many studies suggest that fibrous and multi-channeled scaffolds can potentially be used for nerve regeneration, as the aligned fibers can provide the guidance effect for axonal growth and the mulit-channeled structure mimics the fascicular architecture and decreases the nerve dispersion. A number of studies indicate that concentration gradients of guidance molecules influence axonal growth and cell migration. However, up to now, few studies have combined aligned fibers, mulit-channeled structure and neurotrophic concentration gradients in one integrated system to study their synergistic effect on peripheral nerve regeneration. In this study, aligned fibers were fabricated using an electrospinning technique. The multi-channeled scaffold incorporated with concentration gradient of neurotrophic growth factors was created by a gradient maker. We expect that (i) the aligned fibers can guide the axonal growth to particular direction; (ii) mulit-channeled structure can provide necessary support and decrease the nerve dispersion; (iii) the guidance of molecules gradient can promote axon outgrowth from the proximal stump to distal end.
Conclusions: In this study, we have successfully developed a nerve guidance conduit containing aligned electrospun nanofibers in the multi-channeled gelatin scaffold with the incorporation of neurotrophic gradient. The results show that Rhod-GNs scaffold display a long-term controlled release of encapsulated biomolecules. This pattern suggests the possibility to provide and maintain effective constant concentration release of neurotrophic factors for nerve regeneration. The mechanical property of fabricated nerve conduit is significantly improved after dehydrothermal treatment (DHT) and enzymatic crosslinking (mTG). In in vitro study, differentiated neural stem cells (NSCs) can extend their neurites along the aligned nanofibrous structure. We are now combining theses three stimulating factors together to study their synergistic effect and evaluate the potential for the application in peripheral nerve regeneration.
Bilateral Joint Research Project between Cross-strait Tsing Hua University (104N2705E1); Ministry of Science and Technology (MOST 104-2628-E-007 -002 -MY3, MOST 104-2221-E-007 -140 -MY2)
Keywords:
growth factor,
Scaffold,
Tissue Regeneration,
nanotopography
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Biomaterials in constructing tissue substitutes
Citation:
Wang
T,
Chang
Y,
Wu
H,
Yeh
C and
Chen
M
(2016). Multi-channeled gelatin scaffold incorporated with neurotrophic gradient and nanotopography as nerve guidance conduit for peripheral nerve regeneration.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.01284
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.
*
Correspondence:
Dr. Tzu-Wei Wang, National Tsing Hua University, Department of Materials Science and Engineering, Hsinchu, Taiwan, Email1
Dr. Yo-Cheng Chang, National Tsing Hua University, Department of Materials Science and Engineering, Hsinchu, Taiwan, Email2
Dr. Hsi-Chin Wu, Tatung University, Department of Material Engineering, Taipei, Taiwan, Email3
Dr. Chia-Wei Yeh, National Tsing Hua University, Department of Materials Science and Engineering, Hsinchu, Taiwan, Email4